Co-transfection of decorin and interleukin-10 modulates pro-fibrotic extracellular matrix gene expression in human tenocyte culture

Sci Rep. 2016 Feb 10:6:20922. doi: 10.1038/srep20922.

Abstract

Extracellular matrix synthesis and remodelling are driven by increased activity of transforming growth factor beta 1 (TGF-β1). In tendon tissue repair, increased activity of TGF-β1 leads to progressive fibrosis. Decorin (DCN) and interleukin 10 (IL-10) antagonise pathological collagen synthesis by exerting a neutralising effect via downregulation of TGF-β1. Herein, we report that the delivery of DCN and IL-10 transgenes from a collagen hydrogel system supresses the constitutive expression of TGF-β1 and a range of pro-fibrotic extracellular matrix genes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cells, Cultured
  • Collagen / genetics
  • Collagen / metabolism
  • Decorin / genetics*
  • Decorin / metabolism
  • Elastin / genetics
  • Elastin / metabolism
  • Extracellular Matrix Proteins / genetics*
  • Extracellular Matrix Proteins / metabolism
  • Fibronectins / genetics
  • Fibronectins / metabolism
  • Fibrosis
  • Gene Expression Regulation*
  • Humans
  • Interleukin-10 / genetics*
  • Interleukin-10 / metabolism
  • Tendons / metabolism
  • Tendons / pathology
  • Tenocytes / metabolism*
  • Transfection
  • Transforming Growth Factor beta1 / genetics
  • Transforming Growth Factor beta1 / metabolism

Substances

  • Decorin
  • Extracellular Matrix Proteins
  • Fibronectins
  • Transforming Growth Factor beta1
  • Interleukin-10
  • Collagen
  • Elastin