Abstract
Extracellular matrix synthesis and remodelling are driven by increased activity of transforming growth factor beta 1 (TGF-β1). In tendon tissue repair, increased activity of TGF-β1 leads to progressive fibrosis. Decorin (DCN) and interleukin 10 (IL-10) antagonise pathological collagen synthesis by exerting a neutralising effect via downregulation of TGF-β1. Herein, we report that the delivery of DCN and IL-10 transgenes from a collagen hydrogel system supresses the constitutive expression of TGF-β1 and a range of pro-fibrotic extracellular matrix genes.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cells, Cultured
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Collagen / genetics
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Collagen / metabolism
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Decorin / genetics*
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Decorin / metabolism
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Elastin / genetics
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Elastin / metabolism
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Extracellular Matrix Proteins / genetics*
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Extracellular Matrix Proteins / metabolism
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Fibronectins / genetics
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Fibronectins / metabolism
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Fibrosis
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Gene Expression Regulation*
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Humans
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Interleukin-10 / genetics*
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Interleukin-10 / metabolism
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Tendons / metabolism
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Tendons / pathology
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Tenocytes / metabolism*
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Transfection
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Transforming Growth Factor beta1 / genetics
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Transforming Growth Factor beta1 / metabolism
Substances
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Decorin
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Extracellular Matrix Proteins
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Fibronectins
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Transforming Growth Factor beta1
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Interleukin-10
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Collagen
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Elastin