Abstract
F4(+) enterotoxigenic Escherichia coli (ETEC) strains cause diarrheal disease in neonatal and post-weaned piglets. Several different host receptors for F4 fimbriae have been described, with porcine aminopeptidase N (APN) reported most recently. The FaeG subunit is essential for the binding of the three F4 variants to host cells. Here we show in both yeast two-hybrid and pulldown assays that APN binds directly to FaeG, the major subunit of F4 fimbriae, from three serotypes of F4(+) ETEC. Modulating APN gene expression in IPEC-J2 cells affected ETEC adherence. Antibodies raised against APN or F4 fimbriae both reduced ETEC adherence. Thus, APN mediates the attachment of F4(+) E. coli to intestinal epithelial cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adhesins, Escherichia coli / genetics
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Adhesins, Escherichia coli / metabolism
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Animals
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Antibodies, Bacterial / immunology
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CD13 Antigens / genetics
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CD13 Antigens / metabolism*
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Enterotoxigenic Escherichia coli / physiology*
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Epithelial Cells / enzymology
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Epithelial Cells / immunology
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Epithelial Cells / microbiology
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Escherichia coli Infections / immunology
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Escherichia coli Infections / microbiology
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Escherichia coli Infections / veterinary*
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Fimbriae, Bacterial / physiology*
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Gene Expression
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Intestinal Mucosa / enzymology
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Intestinal Mucosa / immunology
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Intestinal Mucosa / microbiology
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Swine
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Swine Diseases / immunology*
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Swine Diseases / microbiology
Substances
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Adhesins, Escherichia coli
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Antibodies, Bacterial
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FaeG protein, E coli
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CD13 Antigens