Spherical silver nanoparticles in the detection of thermally denatured collagens

Anal Bioanal Chem. 2016 Mar;408(8):1993-6. doi: 10.1007/s00216-016-9330-5. Epub 2016 Feb 4.

Abstract

We have developed a rapid colorimetric method to determine the concentration of denatured collagen in solution, which is based on the collagen-silver nanoparticle corona formation. Using the proposed method, the lowest detectable concentration of denatured collagen protein in a solution of pure collagen was 14.7, 8.5, and 8.6 μg mL(-1) for porcine (PCOL), rat tail (RCOL), and type I human recombinant (HCOL) collagen, respectively.

Keywords: Collagen; Nanoparticles/Nanotechnology; Surface plasmon band.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Collagen / analysis
  • Collagen / chemistry*
  • Colorimetry / economics
  • Colorimetry / methods
  • Humans
  • Metal Nanoparticles / chemistry*
  • Protein Corona / analysis
  • Protein Corona / chemistry
  • Protein Denaturation*
  • Rats
  • Recombinant Proteins / analysis
  • Recombinant Proteins / chemistry
  • Silver / chemistry*
  • Swine
  • Temperature

Substances

  • Protein Corona
  • Recombinant Proteins
  • Silver
  • Collagen