We present a novel method of nonuniformity correction of imaging systems in a wide optical spectral range by applying a radiation source with an unknown and spatially nonhomogeneous radiance or radiance temperature distribution. The benefit of this method is that it can be applied with radiation sources of arbitrary spatial radiance or radiance temperature distribution and only requires the sufficient temporal stability of this distribution during the measurement process. The method is based on the recording of several (at least three) images of a radiation source and a purposeful row- and line-shift of these sequent images in relation to the first primary image. The mathematical procedure is explained in detail. Its numerical verification with a source of a predefined nonhomogenous radiance distribution and a thermal imager of a predefined nonuniform focal plane array responsivity is presented.