Studies on Transcriptional Incorporation of 5'-N-Triphosphates of 5'-Amino-5'-Deoxyribonucleosides

Weronika Kotkowiak; Anna Pasternak; Ryszard Kierzek

doi:10.1371/journal.pone.0148282

Studies on Transcriptional Incorporation of 5'-N-Triphosphates of 5'-Amino-5'-Deoxyribonucleosides

PLoS One. 2016 Feb 1;11(2):e0148282. doi: 10.1371/journal.pone.0148282. eCollection 2016.

Authors

Weronika Kotkowiak¹, Anna Pasternak¹, Ryszard Kierzek¹

Affiliation

¹ Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Poland.

Abstract

In this study, several RNA polymerases were used for the first time to examine the possibility of transcriptional incorporation of 5'-N-triphosphates of 5'-amino-5'-deoxyribonucleosides (5'NH NTPs). The T3, T7, Sp6 and T7 Y639F RNA polymerases were employed to show that the full-length transcript cannot be synthesized. The results suggest that the application of 5'NH NTPs could decrease transcription reaction rates. What is more, the modification of transcription conditions had no influence on the rate of 5'NH NTPs incorporation. Based on experimental data it is postulated that 5'NH NTPs can be used as potential transcription inhibitors. Our findings expand the knowledge on suitable uses of the 5'-N-triphosphates of 5'-amino-5'-deoxyribonucleoside and the exact mechanism of transcriptional inhibition.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA-Directed RNA Polymerases / metabolism
Deoxyribonucleosides / metabolism*
RNA / biosynthesis*
Transcription, Genetic*

Substances

Deoxyribonucleosides
RNA
DNA-Directed RNA Polymerases

Grants and funding

This work has been financed by the National Science Center grant (UMO-2011/03/B/NZ1/00576 to R.K.) and by the Polish Ministry of Science and Higher Education grant (Młod-WK 500-8051 to W.K.). This publication was also supported by the Polish Ministry of Science and Higher Education, under the KNOW program.