Cell survival of glioblastoma grown in medium containing hydrogen peroxide and/or nitrite, or in plasma-activated medium

Arch Biochem Biophys. 2016 Sep 1:605:102-8. doi: 10.1016/j.abb.2016.01.011. Epub 2016 Jan 26.

Abstract

Non-equilibrium atmospheric pressure plasmas generate a high electron density (on the order of 10(16) electrons per cm(-3)) using Ar gas. Culture medium in air at room temperature was plasma-irradiated for several hundred seconds. Tens of micromolar hydrogen peroxide (H2O2) and millimolar levels of nitrous ion (NO2(-)) were detected in the plasma-irradiated culture medium (plasma activated medium; PAM) and selectively induced the apoptotic death of glioblastoma tumor cells, but did not kill normal mammary epithelial cells. A similar antitumor effect was induced by spiking the medium with comparable concentrations of H2O2 and NO2(-). The PAM remained still a somewhat difference that it should also be assessed for understanding other latent mechanisms.

Keywords: Apoptosis; Glioblastoma; Hydrogen peroxide; Nitrites; Non-equilibrium atmospheric pressure plasma; Plasma activated medium.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis
  • Brain Neoplasms / drug therapy
  • Brain Neoplasms / metabolism*
  • Cattle
  • Cell Line, Tumor
  • Cell Survival
  • Culture Media / chemistry*
  • Electrons
  • Glioblastoma / drug therapy
  • Glioblastoma / metabolism*
  • Humans
  • Hydrogen Peroxide / chemistry*
  • Nitrates / chemistry
  • Nitrites / chemistry*
  • Nitrogen Dioxide / chemistry
  • Oxidants / chemistry
  • Plasma Gases*
  • Reactive Nitrogen Species / chemistry
  • Reactive Oxygen Species / chemistry

Substances

  • Culture Media
  • Nitrates
  • Nitrites
  • Oxidants
  • Plasma Gases
  • Reactive Nitrogen Species
  • Reactive Oxygen Species
  • Hydrogen Peroxide
  • Nitrogen Dioxide