Colorimetric detection of bisphenol A based on unmodified aptamer and cationic polymer aggregated gold nanoparticles

Dongwei Zhang; Jiayi Yang; Jing Ye; Lurong Xu; Hanchu Xu; Shenshan Zhan; Bing Xia; Lumei Wang

doi:10.1016/j.ab.2016.01.011

Colorimetric detection of bisphenol A based on unmodified aptamer and cationic polymer aggregated gold nanoparticles

Anal Biochem. 2016 Apr 15:499:51-56. doi: 10.1016/j.ab.2016.01.011. Epub 2016 Jan 25.

Authors

Dongwei Zhang¹, Jiayi Yang², Jing Ye¹, Lurong Xu³, Hanchu Xu³, Shenshan Zhan³, Bing Xia¹, Lumei Wang⁴

Affiliations

¹ School of Chemical and Environmental Engineering, Shanghai Institute of Technology, Shanghai 201418, China.
² School of Agriculture and Biology and Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, and Bor S. Luh Food Safety Research Center, Shanghai Jiao Tong University, Shanghai 200240, China; Department of Management, Shanghai Sanda College, Shanghai 201209, China.
³ School of Agriculture and Biology and Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, and Bor S. Luh Food Safety Research Center, Shanghai Jiao Tong University, Shanghai 200240, China.
⁴ School of Chemical and Environmental Engineering, Shanghai Institute of Technology, Shanghai 201418, China; School of Agriculture and Biology and Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, and Bor S. Luh Food Safety Research Center, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address: zjuwlm@163.com.

PMID: 26820097
DOI: 10.1016/j.ab.2016.01.011

Abstract

In this study, a colorimetric method was exploited to detect bisphenol A (BPA) based on BPA-specific aptamer and cationic polymer-induced aggregation of gold nanoparticles (AuNPs). The principle of this assay is very classical. The aggregation of AuNPs was induced by the concentration of cationic polymer, which is controlled by specific recognition of aptamer with BPA and the reaction of aptamer and cationic polymer forming "duplex" structure. This method enables colorimetric detection of BPA with selectivity and a detection limit of 1.50 nM. In addition, this colorimetric method was successfully used to determine spiked BPA in tap water and river water samples.

Keywords: Biosensor; Bisphenol A; Cationic polymer; Colorimetry; Gold nanoparticles.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aptamers, Nucleotide / chemistry*
Benzhydryl Compounds / analysis*
Cations / chemistry
Colorimetry*
Gold / chemistry*
Metal Nanoparticles / chemistry*
Phenols / analysis*
Polymers / chemistry*

Substances

Aptamers, Nucleotide
Benzhydryl Compounds
Cations
Phenols
Polymers
Gold
bisphenol A