Accuracy of universal polymerase chain reaction (PCR) for detection of bacterial meningitis among suspected patients

Ali Reza Moayedi; Abdolazim Nejatizadeh; Maryam Mohammadian; Mohammad Bagher Rahmati; Vahideh Namardizadeh

doi:10.19082/1609

Accuracy of universal polymerase chain reaction (PCR) for detection of bacterial meningitis among suspected patients

Electron Physician. 2015 Dec 20;7(8):1609-12. doi: 10.19082/1609. eCollection 2015 Dec.

Authors

Ali Reza Moayedi¹, Abdolazim Nejatizadeh², Maryam Mohammadian³, Mohammad Bagher Rahmati⁴, Vahideh Namardizadeh⁵

Affiliations

¹ M.D., Assistant Professor, Department of Pediatric Neurology, Faculty of Medicine, Clinical Research Development Center of Children Hospital, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
² Ph.D. of Genetics, Associate Professor, Department of Genetics, Faculty of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
³ M.D., Pediatric Resident, Department of Pediatrics, Faculty of Medicine, Clinical Research Development Center of Children Hospital, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
⁴ M.D., Assistant Professor, Department of Pediatric Infectious Diseases, Faculty of Medicine, Infectious and tropical diseases research center, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
⁵ B.Sc., Department of Genetics, Faculty of Medicine, Khalije-Fars Molecular Medicine Research Center, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.

PMID: 26816587
PMCID: PMC4725414
DOI: 10.19082/1609

Abstract

Introduction: Central nervous system (CNS) infections are life-threatening diseases caused by viral, bacterial, parasitic and fungal microorganisms. The aim of this study was to determine the accuracy of universal polymerase chain reaction (PCR) for the detection of bacterial meningitis among patients who were referred to Koodakan Hospital in Bandar Abbas because they were suspected of having the disease.

Methods: This study was conducted in 2013 on the patients who were admitted to Bandar Abbas' Koodakan Hospital because they were suspected of having meningitis. A questionnaire, including demographic data, was completed for each patient. Universal PCR, Cerebrospinal fluid (CSF) analysis, and gram staining and cultures were done for all the patients. The data were analyzed using SPSS software.

Results: Among the 100 patients studied 59 (59%) were male and 41 (41%) were female. No patient in our study had a positive smear and culture for meningitis. Among the patients with negative smears and cultures six (6%) had positive universal PCR, and 94 (94%) had negative universal PCR. Based on these results, PCR had 95% specificity and 100% negative predictive value for the prediction of meningitis. In 30 patients (30%), the biochemical analysis of CSF were in favor of meningitis. Among the 30 patients, six patients (20%) had positive universal PCR and 24 patients (80%) had negative universal PCR.

Conclusion: Based on our results, the universal PCR test is useful in the diagnosis of bacterial meningitis in children. We recommend using it in combination with other tests, such as CSF analysis, for diagnosis of bacterial meningitis.

Keywords: gram stain and culture; meningitis; universal PCR.