Abstract
Eleven new abietane-type diterpenes, holophyllins D-N (1-11), and 17 known analogues (12-28), were isolated from a MeOH extract of the trunk of Abies holophylla. The chemical structures of 1-11 were determined through spectroscopic data analysis, including NMR ((1)H and (13)C NMR, DEPT, (1)H-(1)H COSY, HMQC, HMBC, and NOESY) and HRFABMS methods. All isolated compounds (1-28) were evaluated for their cytotoxicity against four human tumor cell lines (A549, SK-OV-3, SK-MEL-2, and HCT-116), for their potential neuroprotective effects through induction of nerve growth factor in C6 glioma cells, and for their effects on nitric oxide levels in lipopolysaccharide-stimulated murine microglia BV2 cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Abies / chemistry*
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Animals
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Antineoplastic Agents, Phytogenic / chemistry
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Antineoplastic Agents, Phytogenic / isolation & purification*
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Antineoplastic Agents, Phytogenic / pharmacology*
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Diterpenes / chemistry
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Diterpenes / isolation & purification*
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Diterpenes / pharmacology*
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Drug Screening Assays, Antitumor
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Humans
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Inhibitory Concentration 50
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Lipopolysaccharides / pharmacology
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Mice
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Microglia / drug effects
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Molecular Structure
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Nerve Growth Factor / drug effects
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Neuroprotective Agents / chemistry
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Neuroprotective Agents / isolation & purification*
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Neuroprotective Agents / pharmacology*
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Nitric Oxide / analysis
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Plant Stems / chemistry
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Republic of Korea
Substances
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Antineoplastic Agents, Phytogenic
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Diterpenes
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Lipopolysaccharides
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Neuroprotective Agents
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Nitric Oxide
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Nerve Growth Factor