Mycobacterium tuberculosis Mutations Associated with Reduced Susceptibility to Linezolid

Shuo Zhang; Jiazhen Chen; Peng Cui; Wanliang Shi; Xiaohong Shi; Hongxia Niu; Denise Chan; Wing Wai Yew; Wenhong Zhang; Ying Zhang

doi:10.1128/AAC.02941-15

Mycobacterium tuberculosis Mutations Associated with Reduced Susceptibility to Linezolid

Antimicrob Agents Chemother. 2016 Mar 25;60(4):2542-4. doi: 10.1128/AAC.02941-15. Print 2016 Apr.

Authors

Shuo Zhang¹, Jiazhen Chen², Peng Cui², Wanliang Shi¹, Xiaohong Shi³, Hongxia Niu⁴, Denise Chan⁵, Wing Wai Yew⁵, Wenhong Zhang², Ying Zhang⁶

Affiliations

¹ Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland, USA.
² Key Lab of Molecular Virology, Institute of Medical Microbiology, Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, China.
³ Department of Laboratory Medicine, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan, Shandong, China.
⁴ Institute of Pathogenic Biology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, China.
⁵ Stanley Ho Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Hong Kong, China.
⁶ Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland, USA Key Lab of Molecular Virology, Institute of Medical Microbiology, Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, China yzhang@jhsph.edu.

Abstract

Linezolid (LZD) has become increasingly important for the treatment of multidrug-resistant tuberculosis (MDR-TB), but its mechanisms of resistance are not well characterized. We isolated 32 mutants ofMycobacterium tuberculosiswith reduced susceptibility to LZD, which was accounted for byrrlandrplCmutations in almost equal proportions, causing lower and higher MICs, respectively. Our findings provide useful information for the rapid detection of LZD resistance for improved treatment of MDR-TB.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Substitution
Antitubercular Agents / pharmacology*
Drug Resistance, Multiple, Bacterial / genetics
Gene Expression
Genes, Bacterial*
Humans
Linezolid / pharmacology*
Microbial Sensitivity Tests
Mutation*
Mycobacterium tuberculosis / drug effects*
Mycobacterium tuberculosis / genetics
Mycobacterium tuberculosis / isolation & purification
Mycobacterium tuberculosis / metabolism
RNA, Ribosomal, 23S / genetics*
RNA, Ribosomal, 23S / metabolism
Ribosomal Protein L3
Ribosomal Proteins / genetics*
Ribosomal Proteins / metabolism
Tuberculosis, Multidrug-Resistant / drug therapy
Tuberculosis, Multidrug-Resistant / microbiology

Substances

Antitubercular Agents
RNA, Ribosomal, 23S
Ribosomal Protein L3
Ribosomal Proteins
Linezolid

Abstract

Publication types

MeSH terms

Substances

Grants and funding