Protein phosphatase magnesium-dependent 1δ is a novel tumor marker and target in hepatocellular carcinoma

Front Med. 2016 Mar;10(1):52-60. doi: 10.1007/s11684-016-0433-3. Epub 2016 Jan 25.

Abstract

Hepatocellular carcinoma (HCC) is a lethal liver malignancy worldwide. In this study, we reported that protein phosphatase magnesium-dependent 1δ (PPM1D) was highly expressed in the majority of HCC cases (approximately 59%) and significantly associated with high serum α-fetoprotein (AFP) level (P = 0.044). Kaplan- Meier and Cox regression data indicated that PPM1D overexpression was an independent predictor of HCCspecific overall survival (HR, 2.799; 95% CI, 1.346-5.818, P = 0.006). Overexpressing PPM1D promoted cell viability and invasion, whereas RNA interference-mediated knockdown of PPM1D inhibited proliferation, invasion, and migration of cultured HCC cells. In addition, PPM1D suppression by small interfering RNA decreased the tumorigenicity of HCC cells in vivo. Overall, results suggest that PPM1D is a potential prognostic marker and therapeutic target for HCC.

Keywords: PPM1D; hepatocellular carcinoma; prognosis; target therapy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biomarkers, Tumor / blood
  • Carcinoma, Hepatocellular / blood
  • Carcinoma, Hepatocellular / diagnosis
  • Carcinoma, Hepatocellular / metabolism*
  • Cell Line, Tumor
  • Female
  • Gene Knockdown Techniques
  • Humans
  • Liver Neoplasms / blood
  • Liver Neoplasms / diagnosis
  • Liver Neoplasms / metabolism*
  • Male
  • Mice, Inbred BALB C
  • Middle Aged
  • Neoplasm Invasiveness
  • Neoplasm Transplantation
  • Prognosis
  • Protein Phosphatase 2C / blood*
  • RNA, Small Interfering
  • Survival Analysis
  • alpha-Fetoproteins / analysis

Substances

  • Biomarkers, Tumor
  • RNA, Small Interfering
  • alpha-Fetoproteins
  • PPM1D protein, human
  • Protein Phosphatase 2C