Development of carboxymethyl cellulose-based hydrogel and nanosilver composite as antimicrobial agents for UTI pathogens

Saad M Alshehri; Ali Aldalbahi; Abdullah Baker Al-Hajji; Anis Ahmad Chaudhary; Marc In Het Panhuis; Norah Alhokbany; Tansir Ahamad

doi:10.1016/j.carbpol.2015.11.004

Development of carboxymethyl cellulose-based hydrogel and nanosilver composite as antimicrobial agents for UTI pathogens

Carbohydr Polym. 2016 Mar 15:138:229-36. doi: 10.1016/j.carbpol.2015.11.004. Epub 2015 Dec 2.

Authors

Saad M Alshehri¹, Ali Aldalbahi², Abdullah Baker Al-Hajji³, Anis Ahmad Chaudhary⁴, Marc In Het Panhuis⁵, Norah Alhokbany⁶, Tansir Ahamad⁷

Affiliations

¹ Department of Chemistry, King Saud University, PO Box 2455, Riyadh 11451, Saudi Arabia. Electronic address: alshehri@ksu.edu.sa.
² Department of Chemistry, King Saud University, PO Box 2455, Riyadh 11451, Saudi Arabia. Electronic address: aaldalbahi@ksu.edu.sa.
³ Department of Chemistry, King Saud University, PO Box 2455, Riyadh 11451, Saudi Arabia. Electronic address: abh121@hotmail.com.
⁴ College of Medicine, Al-Imam Muhammad Ibn Saud Islamic University (IMSIU), Riyadh, Saudi Arabia. Electronic address: anis.chaudhary@gmail.com.
⁵ Soft Materials Group, School of Chemistry, University of Wollongong, Wollongong 2522, NSW, Australia; Intelligent Polymer Research Institute, ARC Centre of Excellence for Electromaterials Science, AIIM Facility, University of Wollongong, Wollongong 2522, NSW, Australia. Electronic address: panhuis@uow.edu.au.
⁶ Department of Chemistry, King Saud University, PO Box 2455, Riyadh 11451, Saudi Arabia. Electronic address: nhokbany@ksu.edu.sa.
⁷ Department of Chemistry, King Saud University, PO Box 2455, Riyadh 11451, Saudi Arabia. Electronic address: tahamed@ksu.edu.sa.

PMID: 26794757
DOI: 10.1016/j.carbpol.2015.11.004

Abstract

Silver nanoparticles (AgNPs) containing hydrogel composite were first synthesized by preparing a new hydrogel from carboxymethyl cellulose (CMC), polyvinyl alcohol (PVA), and the cross-linker ethylene glycol diglycidyl ether (EGDE), followed by the incorporation of AgNPs by microwave radiation. The resulting neat hydrogels and AgNPs-hydrogel composites were characterized using spectral, thermal, microscopic analysis and X-ray diffraction (XRD) analyses. The SEM and TEM results demonstrated that the synthesized AgNPs were spherical with diameters ranging from 8 to 14nm. In addition, the XRD analysis confirmed the nanocrystalline phase of silver with face-centered cubic (FCC) crystal structure. Energy dispersive spectroscopy (EDS) analysis of the AgNPs confirmed the presence of an elemental silver signal, and no peaks of any other impurities were detected. Additionally, the antibacterial activities of the neat hydrogel and AgNPs-hydrogel composites were measured by Kirby-Bauer method against urinary tract infection (UTI) pathogens. The rheology measurement revealed that the values of storage modulus (G') were higher than that of loss modulus (G″). The AgNPs-hydrogel composites exhibited higher antibacterial activity against Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Proteus vulgaris, Staphylococcus aureus and Proteus mirabilis compared to the corresponding neat hydrogel.

Keywords: Antimicrobial activity; FT-IR; Hydrogel; Silver; Urinary tract infection.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Anti-Infective Agents / chemistry*
Anti-Infective Agents / pharmacology
Carboxymethylcellulose Sodium / chemistry*
Gram-Negative Bacteria / drug effects
Gram-Positive Bacteria / drug effects
Humans
Hydrogels / chemistry*
Metal Nanoparticles / chemistry*
Microscopy, Electron, Transmission
Particle Size
Silver / chemistry*
Spectroscopy, Fourier Transform Infrared
Temperature
Thermogravimetry
Urinary Tract Infections / microbiology

Substances

Anti-Infective Agents
Hydrogels
Silver
Carboxymethylcellulose Sodium