Improving the osteogenesis of rat mesenchymal stem cells by chitosan-based-microRNA nanoparticles

Guangsheng Wu; Chao Feng; Guangyan Hui; Zhongshan Wang; Jun Tan; Lankun Luo; Peng Xue; Qintao Wang; Xiguang Chen

doi:10.1016/j.carbpol.2015.11.044

Improving the osteogenesis of rat mesenchymal stem cells by chitosan-based-microRNA nanoparticles

Carbohydr Polym. 2016 Mar 15:138:49-58. doi: 10.1016/j.carbpol.2015.11.044. Epub 2015 Nov 19.

Authors

Guangsheng Wu¹, Chao Feng², Guangyan Hui³, Zhongshan Wang⁴, Jun Tan⁵, Lankun Luo⁵, Peng Xue⁵, Qintao Wang⁶, Xiguang Chen⁷

Affiliations

¹ State Key Laboratory of Military Stomatology, Shaanxi Key Laboratory of Stomatology, Department of Periodontology, School of Stomatology, Fourth Military Medical University, No. 145 West Changle Road, Xi'an 710032, China; College of Marine Life Science, Ocean University of China, No. 5 Yushan Road, Qingdao 266003, Shandong Province, China; Qingdao First Sanatorium, Jinan Military Region, No. 27 West Hong Kong Road, Qingdao 266071, Shandong Province, China.
² College of Marine Life Science, Ocean University of China, No. 5 Yushan Road, Qingdao 266003, Shandong Province, China.
³ Qingdao First Sanatorium, Jinan Military Region, No. 27 West Hong Kong Road, Qingdao 266071, Shandong Province, China.
⁴ State Key Laboratory of Military Stomatology, Department of Prosthetic Dentistry, School of Stomatology, Fourth Military Medical University, No. 145 West Changle Road, Xi'an 710032, China.
⁵ State Key Laboratory of Military Stomatology, Shaanxi Key Laboratory of Stomatology, Department of Periodontology, School of Stomatology, Fourth Military Medical University, No. 145 West Changle Road, Xi'an 710032, China.
⁶ State Key Laboratory of Military Stomatology, Shaanxi Key Laboratory of Stomatology, Department of Periodontology, School of Stomatology, Fourth Military Medical University, No. 145 West Changle Road, Xi'an 710032, China. Electronic address: yznmbk@fmmu.edu.cn.
⁷ College of Marine Life Science, Ocean University of China, No. 5 Yushan Road, Qingdao 266003, Shandong Province, China. Electronic address: xgchen@ouc.edu.cn.

PMID: 26794737
DOI: 10.1016/j.carbpol.2015.11.044

Abstract

MicroRNAs (miRNAs) play important roles in the osteogenic differentiation of stem cells. However, the application of miRNA in bone regeneration has been limited by its poor stability, low cellular uptake, and undesired immune response. In this study, chitosan (CS)/tripolyphosphate (TPP)/Hyaluronic Acid (HA) nanoparticles (CTH NPs) were prepared to deliver antimiR-138 to bone marrow mesenchymal stem cells (MSCs). The particle size, polydispersity index, and zeta potential of CTH NPs were related to the weight ratio of CS:TPP:HA. At optimum N/P ratio (20:1), the highest encapsulation efficiency was obtained. Both blank CTH NPs and CTH/antmiR-138 NPs exhibited no cytotoxicity to MSCs. A high transfection efficiency (nearly 70%) and significant enhancement of the osteogenesis of MSCs were observed. Above results demonstrated that CTH NPs was a potential candidate as an efficient non-viral miRNA vector to regulate the osteogenic differentiation of MSCs.

Keywords: Chitosan; Chitosan (PubChem CID: 21896651); Hyaluronic acid (PubChem CID: 24728612); Mesenchymal stem cells; Nanoparticle; Osteogenic differentiation; microRNA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antigens, CD / metabolism
Cell Differentiation / drug effects
Cell Survival / drug effects
Chitosan / chemistry*
Drug Carriers / chemistry*
Drug Carriers / toxicity
Hyaluronic Acid / chemistry
Mesenchymal Stem Cells / cytology*
Mesenchymal Stem Cells / metabolism
MicroRNAs / metabolism*
Microscopy, Fluorescence
Nanoparticles / chemistry*
Osteogenesis / drug effects
Particle Size
Polyphosphates / chemistry
Rats
Transfection

Substances

Antigens, CD
Drug Carriers
MicroRNAs
Polyphosphates
Hyaluronic Acid
Chitosan
triphosphoric acid