Mass spectrometric detection of biomarkers for early assessment of intraamniotic fluid infection

Consuelo Cháfer-Pericás; Vedran Stefanovic; Ángel Sánchez-Illana; Javier Escobar; María Cernada; Elena Cubells; Antonio Núñez-Ramiro; Sture Andersson; Máximo Vento; Julia Kuligowski

doi:10.1016/j.dib.2015.11.020

Mass spectrometric detection of biomarkers for early assessment of intraamniotic fluid infection

Data Brief. 2015 Nov 20:5:1026-30. doi: 10.1016/j.dib.2015.11.020. eCollection 2015 Dec.

Affiliations

¹ Neonatal Research Unit, Health Research Institute La Fe, Valencia, Avenida Fernando Abril Martorell 106, 46026 Valencia, Spain.
² Department of Obstetrics and Gynecology, Fetomaternal Medical Center, Helsinki University Central Hospital and University of Helsinki, Haartmaninkatu 2, 00029 Helsinki, Finland.
³ Children׳s Hospital, University of Helsinki and Helsinki University Hospital, Stenbäckinkatu 11, PO Box 281, 00029 Helsinki, Finland.
⁴ Neonatal Research Unit, Health Research Institute La Fe, Valencia, Avenida Fernando Abril Martorell 106, 46026 Valencia, Spain; Division of Neonatology, University & Polytechnic Hospital La Fe, Avenida Fernando Abril Martorell 106, 46026 Valencia, Spain.

Abstract

This data article contains information on glutathione sulfonamide (GSA) structural confirmation and purity after synthesis, as well as mass spectrometry acquisition parameters for the determination of GSA and other biomarkers for the early assessment of intraamniotic fluid infection in amniotic fluid samples (Cháfer-Pericás et al., 2015) [1]. GSA standards were synthesized and structural confirmation was carried out employing time-of-flight mass spectrometry (TOF-MS); purity was assessed by high performance liquid chromatography (HPLC) with UV detection. For optimization of the acquisition parameters of GSA and other biomarkers, individual analytical standard solution at a concentration of 1 µmol L(-) (1) was injected into an Acquity - Xevo TQ liquid-chromatography coupled to tandem mass spectrometry (LC-MS/MS) system from Waters (Milford, MA, USA) operating in the positive electrospray (ESI(+)) mode. Mass spectrometric detection of 3-nitro-tyrosine (3NO2-Tyr), 3-chloro-tyrosine (3Cl-Tyr), 8-hydroxy-2'-deoxyguanosine (8OHdG), GSA and oxidized glutathione (GSSG) was carried out by multiple reaction monitoring (MRM). Linear response curves were calculated for each analyte normalizing the signal with peak areas of internal standards.

Keywords: Biomarker detection; Glutathione sulfonamide (GSA); High performance liquid chromatography (HPLC); Intraamniotic fluid infection; Liquid-chromatography coupled to tandem mass spectrometry (LC-MS/MS); Time-of-flight mass spectrometry (TOF-MS).