Abstract
The treatment ofStenotrophomonas maltophiliainfection with β-lactam antibiotics leads to increased release of outer membrane vesicles (OMVs), which are packed with two chromosomally encoded β-lactamases. Here, we show that these β-lactamase-packed OMVs are capable of establishing extracellular β-lactam degradation. We also show that they dramatically increase the apparent MICs of imipenem and ticarcillin for the cohabituating speciesPseudomonas aeruginosaandBurkholderia cenocepacia.
Copyright © 2016, American Society for Microbiology. All Rights Reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Bacterial Agents / pharmacology
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Burkholderia cenocepacia / drug effects
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Burkholderia cenocepacia / enzymology
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Burkholderia cenocepacia / genetics*
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Cell Membrane / chemistry
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Conjugation, Genetic
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Extracellular Vesicles / chemistry
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Extracellular Vesicles / enzymology*
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Gene Expression
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Gene Transfer, Horizontal
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Hydrolysis
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Imipenem / pharmacology
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Microbial Sensitivity Tests
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Pseudomonas aeruginosa / drug effects
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Pseudomonas aeruginosa / enzymology
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Pseudomonas aeruginosa / genetics*
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Stenotrophomonas maltophilia / drug effects
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Stenotrophomonas maltophilia / enzymology
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Stenotrophomonas maltophilia / genetics*
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Ticarcillin / pharmacology
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beta-Lactam Resistance / genetics*
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beta-Lactamases / genetics
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beta-Lactamases / metabolism*
Substances
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Anti-Bacterial Agents
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Imipenem
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beta-Lactamases
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Ticarcillin