Brackish water irrigation utilization is an important way to alleviate water resource shortage in arid region. A field-plot experiment was set up to study the impact of the salinity level (0.31, 3.0 or 5.0 g · L(-1) NaCl) of irrigated water on activities of soil catalase, invertase, β-glucosidase, cellulase and polyphenoloxidase in drip irrigation condition, and the responses of soil CO2 flux and organic matter decomposition were also determined by soil carbon dioxide flux instrument (LI-8100) and nylon net bag method. The results showed that in contrast with fresh water irrigation treatment (CK), the activities of invertase, β-glucosidase and cellulase in the brackish water (3.0 g · L(-1)) irrigation treatment declined by 31.7%-32.4%, 29.7%-31.6%, 20.8%-24.3%, respectively, while soil polyphenoloxidase activity was obviously enhanced with increasing the salinity level of irrigated water. Compared to CK, polyphenoloxidase activity increased by 2.4% and 20.5%, respectively, in the brackish water and saline water irrigation treatments. Both soil microbial biomass carbon and microbial quotient decreased with increasing the salinity level, whereas, microbial metabolic quotient showed an increasing tendency with increasing the salinity level. Soil CO2 fluxes in the different treatments were in the order of CK (0.31 g · L(-1)) > brackish water irrigation (3.0 g · L(-1)) ≥ saline water irrigation (5.0 g · L(-1)). Moreover, CO2 flux from plastic film mulched soil was always much higher than that from no plastic film mulched soil, regardless the salinity of irrigated water. Compared with CK, soil CO2 fluxes in the saline water and brackish water treatments decreased by 29.8% and 28.2% respectively in the boll opening period. The decomposition of either cotton straw or alfalfa straw in the different treatments was in the sequence of CK (0.31 g · L(-1)) > brackish water irrigation (3.0 g · L(-1)) > saline water treatment (5.0 g · L(-1)). The organic matter decomposition rate in the plastic film mulched soil was significantly higher than that in the no plastic film mulched soil. 125 days after incubation, the recovery rates of cotton straw and alfalfa straw were 39.7% and 46.5% with saline water irrigation, 36.3% and 36.5% with brackish water irrigation, and 30.5% and 35.4% with CK, respectively. In conclusion, brackish water drip irrigation had a significant adverse effect on soil enzyme activities, which decreased soil microbial biomass, soil CO2 flux and soil organic matter decomposition, and subsequently deteriorated the soil biological characteristics in oasis farmland.