Complete genome sequence of cyanobacterium Fischerella sp. NIES-3754, providing thermoresistant optogenetic tools

Yuu Hirose; Takatomo Fujisawa; Yoshiyuki Ohtsubo; Mitsunori Katayama; Naomi Misawa; Sachiko Wakazuki; Yohei Shimura; Yasukazu Nakamura; Masanobu Kawachi; Hirofumi Yoshikawa; Toshihiko Eki; Yu Kanesaki

doi:10.1016/j.jbiotec.2016.01.011

Complete genome sequence of cyanobacterium Fischerella sp. NIES-3754, providing thermoresistant optogenetic tools

J Biotechnol. 2016 Feb 20:220:45-6. doi: 10.1016/j.jbiotec.2016.01.011. Epub 2016 Jan 16.

Authors

Affiliations

¹ Department of Environmental and Life Sciences, Toyohashi University of Technology, 1-1 Hibarigaoka, Tempaku, Toyohashi, Aichi 441-8580, Japan; Electronics-Inspired Interdisciplinary Research Institute (EIIRIS), Toyohashi University of Technology, 1-1 Hibarigaoka, Tempaku, Toyohashi, Aichi 441-8580, Japan. Electronic address: hirose@ens.tut.ac.jp.
² Center for Information Biology, National Institute of Genetics, Research Organization of Information and Systems, Yata, Mishima 411-8540, Japan.
³ College of Industrial Technology, Nihon University, 2-11-1 Shinei, Narashino, Chiba 275-8576, Japan.
⁴ Department of Environmental Life Sciences, Graduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Sendai, Miyagi 980-8577, Japan.
⁵ Department of Environmental and Life Sciences, Toyohashi University of Technology, 1-1 Hibarigaoka, Tempaku, Toyohashi, Aichi 441-8580, Japan.
⁶ Electronics-Inspired Interdisciplinary Research Institute (EIIRIS), Toyohashi University of Technology, 1-1 Hibarigaoka, Tempaku, Toyohashi, Aichi 441-8580, Japan.
⁷ Center for Environmental Biology and Ecosystem Studies, National Institute for Environmental Studies, Tsukuba, Ibaraki 305-8506, Japan.
⁸ Department of Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan.
⁹ Genome Research Center, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan. Electronic address: y3kanesa@nodai.ac.jp.

PMID: 26784989
DOI: 10.1016/j.jbiotec.2016.01.011

Abstract

Cyanobacterial phytochrome-class photosensors are recently emerging optogenetic tools. We isolated Fischerella sp. strain NIES-3754 from hotspring at Suwa-shrine, Suwa, Nagano, Japan. We determined complete genome sequence of the NIES-3754 strain, which is composed of one chromosome and two putative replicons (total 5,826,863bp containing no gaps). We identified photosensor genes of 5 phytochromes and 9 cyanobacteriochromes, which will facilitate optogenetics of thermophile.

Keywords: Cyanobacteria; Genome; Photosensor; Thermophile.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Proteins / genetics*
Base Composition
Base Sequence*
Chromosome Mapping
Cyanobacteria / classification
Cyanobacteria / genetics*
Cyanobacteria / isolation & purification
Genome, Bacterial*
Hot Springs
Hot Temperature
Japan
Molecular Sequence Data
Optogenetics*
Photoreceptors, Microbial / genetics
Phytochrome / genetics
RNA, Ribosomal / genetics

Substances

Bacterial Proteins
Photoreceptors, Microbial
RNA, Ribosomal
Phytochrome