This study described the utility of green analytical chemistry in the synthesis of gelatin-capped silver, gold and bimetallic gold-silver nanoparticles (NPs). The preparation of nanoparticles was based on the reaction of silver nitrate or chlorauric acid with a 1.0 wt% aqueous gelatin solution at 50°C. The gelatin-capped silver, gold and bimetallic NPs were characterized using transmission electron microscopy, UV-vis, X-ray diffraction and Fourier transform infrared spectroscopy, and were used to enhance a sensitive sequential injection chemiluminescence luminol-potassium ferricyanide system for determination of the anticancer drug raloxifene hydrochloride. The developed method is eco-friendly and sensitive for chemiluminescence detection of the selected drug in its bulk powder, pharmaceutical injections and biosamples. After optimizing the conditions, a linear relationship in the range of 1.0 × 10(-9) to 1.0 × 10(-1) mol/L was obtained with a limit of detection of 5.0 × 10(-10) mol/L and a limit of quantification of 1.0 × 10(-9) mol/L. Statistical treatment and method validation were performed based on ICH guidelines. Copyright © 2016 John Wiley & Sons, Ltd.
Keywords: biosamples; chemiluminescence; gold-silver nanoparticles; green analytical chemistry; raloxifene hydrochloride.
Copyright © 2016 John Wiley & Sons, Ltd.