Objective: To investigate the association between anxiety-depression and 5-HTTLPR gene polymorphism in school-aged twins.
Methods: A total of 147 pairs of twins (47 pairs of monozygotic twins, 100 pairs of dizygotic twins) aged 8-12 years from Baotou and Hohhot were selected as respondents. The Achenbach Child Behavior Checklist (CBCL) was used to calculate the scores of anxiety-depression factors in school-aged twins. The DNA was extracted from oral epithelial cells, and polymerase chain reaction was applied for 5-HTTLPR genotyping. The generalized estimating equation (GEE) was used to analyze the effect of 5-HTTLPR polymorphism and family environment on anxiety-depression in school-aged twins.
Results: The children with LS and SS genotypes had significantly higher scores of anxiety-depression factors than those with LL genotype (χ2=3.938, P<0.05). The interaction of 5-HTTLPR genotype with family cohesion and family rearing patterns had a significant impact on the scores of anxiety-depression factors in twins (χ2=6.129 and 7.665, both P<0.05).
Conclusions: 5-HTTLPR genotype is significantly correlated with the scores of anxiety-depression factors in school-aged twins. In the family with high cohesion and an autocratic family rearing pattern, S allele may increase the possibility of anxiety-depression in twin children.
目的: 了解5-HTTLPR基因多态性与学龄双生子儿童焦虑抑郁的关系。
方法: 选取内蒙古呼和浩特市和包头市8~12岁147对双生子儿童(同卵双生子47对; 异卵双生子100对)为调查对象, 运用Achenbach儿童行为量表(CBCL)测算儿童焦虑抑郁因子得分; 采集所有儿童口腔上皮细胞并提取DNA, 运用聚合酶链式反应(PCR)方法对5-HTTLPR基因进行分型; 利用广义估计方程(GEE)分析5-HTTLPR基因多态性及家庭环境因素对学龄双生子儿童焦虑抑郁的影响。
结果: LS和SS基因型儿童的焦虑抑郁因子得分高于LL基因型儿童(χ2=3.938, P < 0.05);5-HTTLPR基因型与家庭亲密度(χ2=6.129, P < 0.05)和家庭教养方式(χ2=7.665, P < 0.05)的交互作用对儿童焦虑抑郁因子得分影响显著。
结论: 5-HTTLPR基因型与学龄双生子儿童焦虑抑郁因子得分显著相关; 在高亲密度和专制型教养方式的家庭环境中, S等位基因可能增加双生子儿童焦虑抑郁的可能性。