Bioaffinity Mass Spectrometry Screening

J Biomol Screen. 2016 Feb;21(2):194-200. doi: 10.1177/1087057115622605.

Abstract

Electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (ESI-FTICR-MS or ESI-FTMS) was used to screen 192 natural product extracts and a 659-member natural product-based fragment library for bindings to a potential malaria drug target, Plasmodium falciparum Rab11a (PfRab11a, PF13_0119). One natural product extract and 11 fragments showed binding activity. A new natural product, arborside E, was identified from the active extract of Psydrax montigena as a weak binder. Its binding activity and inhibitory activity against PfRab11a were confirmed by ESI-FTMS titration experiments and an orthogonal enzyme assay.

Keywords: FTMS; PfRab11a; malaria.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biological Products / chemistry*
  • Plant Extracts / chemistry
  • Plants / chemistry
  • Spectrometry, Mass, Electrospray Ionization / methods

Substances

  • Biological Products
  • Plant Extracts