A review on recent developments for biomolecule separation at analytical scale using microfluidic devices

Kishore K R Tetala; M A Vijayalakshmi

doi:10.1016/j.aca.2015.11.037

A review on recent developments for biomolecule separation at analytical scale using microfluidic devices

Anal Chim Acta. 2016 Feb 4:906:7-21. doi: 10.1016/j.aca.2015.11.037. Epub 2015 Dec 17.

Authors

Kishore K R Tetala¹, M A Vijayalakshmi²

Affiliations

¹ Advanced Centre for Bioseparation Technology (CBST), VIT University, Vellore, 632014, Tamil Nadu, India. Electronic address: kishore.tetala@gmail.com.
² Advanced Centre for Bioseparation Technology (CBST), VIT University, Vellore, 632014, Tamil Nadu, India.

PMID: 26772122
DOI: 10.1016/j.aca.2015.11.037

Abstract

Microfluidic devices with their inherent advantages like the ability to handle 10(-9) to 10(-18) L volume, multiplexing of microchannels, rapid analysis and on-chip detection are proving to be efficient systems in various fields of life sciences. This review highlights articles published since 2010 that reports the use of microfluidic devices to separate biomolecules (DNA, RNA and proteins) using chromatography principles (size, charge, hydrophobicity and affinity) along with microchip capillary electrophoresis, isotachophoresis etc. A detailed overview of stationary phase materials and the approaches to incorporate them within the microchannels of microchips is provided as well as a brief overview of chemical methods to immobilize ligand(s). Furthermore, we review research articles that deal with microfluidic devices as analytical tools for biomolecule (DNA, RNA and protein) separation.

Keywords: Biomolecule separation; Chromatography; Ligand immobilization; Microfluidic devices; Monoliths.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Ligands
Microfluidic Analytical Techniques / instrumentation*
Surface Properties

Substances

Ligands