Caffeine and REM sleep deprivation: Effect on basal levels of signaling molecules in area CA1

Karim A Alkadhi; Ibrahim A Alhaider

doi:10.1016/j.mcn.2015.12.015

Caffeine and REM sleep deprivation: Effect on basal levels of signaling molecules in area CA1

Mol Cell Neurosci. 2016 Mar:71:125-31. doi: 10.1016/j.mcn.2015.12.015. Epub 2016 Jan 6.

Authors

Karim A Alkadhi¹, Ibrahim A Alhaider²

Affiliations

¹ Department of Pharmacological and Pharmaceutical Sciences, College of Pharmacy, University of Houston, TX, USA. Electronic address: kalkadhi@uh.edu.
² Department of Pharmacological and Pharmaceutical Sciences, College of Pharmacy, University of Houston, TX, USA.

PMID: 26767416
DOI: 10.1016/j.mcn.2015.12.015

Abstract

We have investigated the neuroprotective effect of chronic caffeine treatment on basal levels of memory-related signaling molecules in area CA1 of sleep-deprived rats. Animals in the caffeine groups were treated with caffeine in drinking water (0.3g/l) for four weeks before they were REM sleep-deprived for 24h in the Modified Multiple Platforms paradigm. Western blot analysis of basal protein levels of plasticity- and memory-related signaling molecules in hippocampal area CA1 showed significant down regulation of the basal levels of phosphorylated- and total-CaMKII, phosphorylated- and total-CREB as well as those of BDNF and CaMKIV in sleep deprived rats. All these changes were completely prevented in rats that chronically consumed caffeine. The present findings suggest an important neuroprotective property of caffeine in sleep deprivation.

Keywords: BDNF; CREB; Immunoblot analysis; Modified multiple platforms; Rat.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Brain-Derived Neurotrophic Factor / genetics
Brain-Derived Neurotrophic Factor / metabolism
CA1 Region, Hippocampal / drug effects*
CA1 Region, Hippocampal / metabolism
CA1 Region, Hippocampal / physiology
Caffeine / pharmacology*
Caffeine / therapeutic use
Calcium-Calmodulin-Dependent Protein Kinase Type 2 / genetics
Calcium-Calmodulin-Dependent Protein Kinase Type 2 / metabolism
Central Nervous System Stimulants / pharmacology*
Central Nervous System Stimulants / therapeutic use
Cyclic AMP Response Element-Binding Protein / genetics
Cyclic AMP Response Element-Binding Protein / metabolism
Down-Regulation
Male
Neuroprotective Agents / pharmacology*
Neuroprotective Agents / therapeutic use
Rats
Rats, Wistar
Signal Transduction*
Sleep Deprivation / drug therapy
Sleep Deprivation / metabolism*
Sleep, REM / drug effects*

Substances

Brain-Derived Neurotrophic Factor
Central Nervous System Stimulants
Cyclic AMP Response Element-Binding Protein
Neuroprotective Agents
Caffeine
Calcium-Calmodulin-Dependent Protein Kinase Type 2