Graphene quantum dots FRET based sensor for early detection of heart attack in human

Biosens Bioelectron. 2016 May 15:79:495-9. doi: 10.1016/j.bios.2015.12.083. Epub 2015 Dec 25.

Abstract

Cardiac immunosensor for early detection of heart attack (myocardial infarction) was developed using amine functionalized graphene quantum dots (afGQDs) conjugated with antibody anti-cardiac Troponin I (anti-cTnI) to detect cardiac marker antigen Troponin I (cTnI) in blood based on fluorescence resonance energy transfer (FRET) between conjugate and graphene (quencher) only in 10 min. The anti-cTnI was covalently conjugated to afGQDs through carbodiimide coupling reaction. The conjugate was characterized by zeta potential UV-vis spectroscopy and field emission scanning electron microscopy (FESEM). The sensing performance of the sensor was studied with respect to changes in the photon count and photoluminescence of GQDs based on interaction of target cTnI with its specific anti-cTnI antibody. The sensor is highly specific and shows negligible response to non-specific antigens. The sensor displayed a linear response to cTnI from 0.001 to 1000 ng mL(-1) with a limit of detection of 0.192 pg mL(-1).

Keywords: Cardiac Troponin I sensor; FRET; Graphene quantum dots; Myocardial infarction.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Immobilized / chemistry*
  • Early Diagnosis
  • Fluorescence Resonance Energy Transfer / methods*
  • Graphite / chemistry
  • Humans
  • Immunoassay / methods
  • Limit of Detection
  • Myocardial Infarction / blood*
  • Myocardial Infarction / diagnosis*
  • Quantum Dots / chemistry*
  • Troponin I / blood*

Substances

  • Antibodies, Immobilized
  • Troponin I
  • Graphite