Sorting nexin 27 interacts with Fzd7 and mediates Wnt signalling

Biosci Rep. 2016 Jan 7;36(1):e00296. doi: 10.1042/BSR20150205.

Abstract

SNX27 is the only sorting nexin (SNX) that contains a PDZ domain, which interacts with PDZ-binding motif of target proteins to regulate the trafficking of these proteins. We here showed that SNX27 interacts with Frizzled (Fzd) receptors via PDZ domain interaction. Immunofluorescence microscopy revealed that Fzd7 can be internalized and associate with SNX27-containing endosomal membrane. In addition, SNX27 enhances the endocytosis of Fzd7 and promotes the degradation of Fzd7. Further examination demonstrated that SNX27 inhibits the Wnt regulated transcription activity of TCF/LEF. Our results suggested that SNX27 interacts with Frizzled receptors to regulate the endocytosis and stability of Fzds, and consequently mediates canonical Wnt signalling.

Keywords: Fzd7; PDZ domain; SNX27; Wnt signalling.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Endosomes / genetics
  • Endosomes / metabolism*
  • Frizzled Receptors / genetics
  • Frizzled Receptors / metabolism*
  • HeLa Cells
  • Humans
  • Intracellular Membranes / metabolism*
  • MCF-7 Cells
  • Protein Transport / physiology
  • Sorting Nexins / genetics
  • Sorting Nexins / metabolism*
  • Wnt Signaling Pathway / physiology*

Substances

  • FZD7 protein, human
  • Frizzled Receptors
  • SNX27 protein, human
  • Sorting Nexins