Multiplex RT-PCR detection of H3N2 influenza A virus in dogs

Mol Cell Probes. 2016 Feb;30(1):56-60. doi: 10.1016/j.mcp.2015.12.002. Epub 2015 Dec 29.

Abstract

A multiplex RT-PCR (mRT-PCR) assay to detect H3N2 CIV genomic segments was developed as a rapid and cost-effective method. Its performance was evaluated with forty-six influenza A viruses from different hosts using three primer sets which amplify four segments of H3N2 CIV simultaneously. The mRT-PCR has been successful in detecting the viral segments, indicating that it can improve the speed of diagnosis for H3N2 CIV and its reassortants.

Keywords: H3N2 CIV; Multiplex RT-PCR; Reassortants.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cost-Benefit Analysis
  • Dog Diseases / diagnosis
  • Dog Diseases / virology*
  • Dogs
  • Genome, Viral / genetics
  • Humans
  • Influenza A Virus, H3N2 Subtype / genetics*
  • Orthomyxoviridae Infections / diagnosis
  • Orthomyxoviridae Infections / veterinary*
  • Orthomyxoviridae Infections / virology
  • RNA, Viral / genetics
  • Reproducibility of Results
  • Reverse Transcriptase Polymerase Chain Reaction / economics
  • Reverse Transcriptase Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity

Substances

  • RNA, Viral