Cells and tissues are extremely sensitive to their physico-chemical surroundings: in regenerative medicine, as an example, the maintenance of culture conditions resembling the in vivo environment is essential for a correct tissue development. In this review, we summarize our results concerning the preparation and testing of micro/nanostructures for fostering peculiar cellular behavior, prepared by exploiting an innovative technique of high resolution 3D photolithography, the two-photon polymerization (2pp).