Evaluation of safety and efficacy of adipose-derived stem cells in rat myocardial infarction model using hexadecyl-4-[¹²⁴I]iodobenzoate for cell tracking

Appl Radiat Isot. 2016 Feb:108:116-123. doi: 10.1016/j.apradiso.2015.12.039. Epub 2015 Dec 17.

Abstract

This study was aimed to evaluate the effect of (124)I-labeling with hexadecyl-4-iodobenzoate (HIB) on gene expression related to cell cycle, DNA repair, transcription, proliferation and differentiation of adipose-derived stem cells (ADSCs). [(124)I]HIB showed high labeling efficiency with ADSCs (51.3±1.3%, 0.3-2.0 Bq/cell) and there is no morphological change of ADSCs. In the microarray analysis of gene expression pattern, differences were not observed between non-labeled and [(124)I]HIB-labeled ADSCs. We demonstrated that (124)I-labeling with HIB did not affect the biological properties of ADSCs.

Keywords: Adipose-derived stem cells; Direct labeling agent; Hexadecyl-4-[(124)I]iodobenzoate; Microarray; Myocardial infarction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipocytes / diagnostic imaging
  • Adipocytes / drug effects
  • Adipocytes / transplantation
  • Animals
  • Cell Tracking / methods*
  • Cells, Cultured
  • Iodine Radioisotopes / adverse effects
  • Iodobenzoates / adverse effects*
  • Isotope Labeling / methods
  • Male
  • Myocardial Infarction / diagnostic imaging*
  • Myocardial Infarction / therapy*
  • Positron-Emission Tomography / methods
  • Radiopharmaceuticals / adverse effects
  • Rats
  • Rats, Sprague-Dawley
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Stem Cell Transplantation / adverse effects
  • Stem Cell Transplantation / methods*
  • Stem Cells / diagnostic imaging*
  • Stem Cells / drug effects
  • Treatment Outcome

Substances

  • Iodine Radioisotopes
  • Iodobenzoates
  • Radiopharmaceuticals