Abstract
Recently, there are tendency to use natural products such as Ziziphus Jujube (Jujube) as therapeutic agents for cancer. Understanding the molecular mechanisms of anti-cancer effects of Jujube may improve the current therapeutic strategies against cervical cancer. Our MTT data showed a significant dose- and time-dependent inhibition of OV-2008 cell proliferation following Jujube administration. Moreover, qRT-PCR analyses significantly revealed the suppression of cyclin D1 and the enhancement of P53, P21 and P27 expression in treated cells. These results suggest that the herb exerts a cytotoxic effect on cervical cancer cells through alternation of the expression of the genes that are involved in regulation of cell cycle.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antineoplastic Agents, Phytogenic / isolation & purification
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Antineoplastic Agents, Phytogenic / pharmacology*
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Cell Cycle / drug effects*
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Cell Cycle / genetics
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Cell Line, Tumor
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Cell Proliferation / drug effects
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Cervix Uteri / drug effects
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Cervix Uteri / metabolism
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Cervix Uteri / pathology
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Cyclin D1 / antagonists & inhibitors
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Cyclin D1 / genetics
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Cyclin D1 / metabolism
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Cyclin-Dependent Kinase Inhibitor p21 / agonists
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Cyclin-Dependent Kinase Inhibitor p21 / genetics
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Cyclin-Dependent Kinase Inhibitor p21 / metabolism
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Cyclin-Dependent Kinase Inhibitor p27 / agonists
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Cyclin-Dependent Kinase Inhibitor p27 / genetics
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Cyclin-Dependent Kinase Inhibitor p27 / metabolism
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Dose-Response Relationship, Drug
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Epithelial Cells / drug effects*
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Epithelial Cells / metabolism
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Epithelial Cells / pathology
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Female
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Fruit / chemistry
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Gene Expression Regulation, Neoplastic / drug effects*
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Humans
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Plant Extracts / chemistry
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Signal Transduction
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Tumor Suppressor Protein p53 / agonists
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Tumor Suppressor Protein p53 / genetics
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Tumor Suppressor Protein p53 / metabolism
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Ziziphus / chemistry*
Substances
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Antineoplastic Agents, Phytogenic
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CCND1 protein, human
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Cyclin-Dependent Kinase Inhibitor p21
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Plant Extracts
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Tumor Suppressor Protein p53
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Cyclin D1
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Cyclin-Dependent Kinase Inhibitor p27