The cytotoxic activity of Ziziphus Jujube on cervical cancer cells: In Vitro study

Cell Mol Biol (Noisy-le-grand). 2015 Dec 30;61(8):128-30.

Abstract

Recently, there are tendency to use natural products such as Ziziphus Jujube (Jujube) as therapeutic agents for cancer. Understanding the molecular mechanisms of anti-cancer effects of Jujube may improve the current therapeutic strategies against cervical cancer. Our MTT data showed a significant dose- and time-dependent inhibition of OV-2008 cell proliferation following Jujube administration. Moreover, qRT-PCR analyses significantly revealed the suppression of cyclin D1 and the enhancement of P53, P21 and P27 expression in treated cells. These results suggest that the herb exerts a cytotoxic effect on cervical cancer cells through alternation of the expression of the genes that are involved in regulation of cell cycle.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antineoplastic Agents, Phytogenic / isolation & purification
  • Antineoplastic Agents, Phytogenic / pharmacology*
  • Cell Cycle / drug effects*
  • Cell Cycle / genetics
  • Cell Line, Tumor
  • Cell Proliferation / drug effects
  • Cervix Uteri / drug effects
  • Cervix Uteri / metabolism
  • Cervix Uteri / pathology
  • Cyclin D1 / antagonists & inhibitors
  • Cyclin D1 / genetics
  • Cyclin D1 / metabolism
  • Cyclin-Dependent Kinase Inhibitor p21 / agonists
  • Cyclin-Dependent Kinase Inhibitor p21 / genetics
  • Cyclin-Dependent Kinase Inhibitor p21 / metabolism
  • Cyclin-Dependent Kinase Inhibitor p27 / agonists
  • Cyclin-Dependent Kinase Inhibitor p27 / genetics
  • Cyclin-Dependent Kinase Inhibitor p27 / metabolism
  • Dose-Response Relationship, Drug
  • Epithelial Cells / drug effects*
  • Epithelial Cells / metabolism
  • Epithelial Cells / pathology
  • Female
  • Fruit / chemistry
  • Gene Expression Regulation, Neoplastic / drug effects*
  • Humans
  • Plant Extracts / chemistry
  • Signal Transduction
  • Tumor Suppressor Protein p53 / agonists
  • Tumor Suppressor Protein p53 / genetics
  • Tumor Suppressor Protein p53 / metabolism
  • Ziziphus / chemistry*

Substances

  • Antineoplastic Agents, Phytogenic
  • CCND1 protein, human
  • Cyclin-Dependent Kinase Inhibitor p21
  • Plant Extracts
  • Tumor Suppressor Protein p53
  • Cyclin D1
  • Cyclin-Dependent Kinase Inhibitor p27