Bioanalytical advances in assays for C-reactive protein

Sandeep Kumar Vashist; A G Venkatesh; E Marion Schneider; Christopher Beaudoin; Peter B Luppa; John H T Luong

doi:10.1016/j.biotechadv.2015.12.010

Bioanalytical advances in assays for C-reactive protein

Biotechnol Adv. 2016 May-Jun;34(3):272-90. doi: 10.1016/j.biotechadv.2015.12.010. Epub 2015 Dec 21.

Authors

Sandeep Kumar Vashist¹, A G Venkatesh², E Marion Schneider³, Christopher Beaudoin⁴, Peter B Luppa⁴, John H T Luong⁵

Affiliations

¹ Institute of Clinical Chemistry and Pathobiochemistry, Klinikum rechts der Isar der Technische Universität München, Ismaninger Str. 22, D-81675 Munich, Germany. Electronic address: sandeep.vashist@tum.de.
² Department of Electrical and Computer Engineering, Jacobs School of Engineering, University of California San Diego, San Diego, CA 92093, U.S.A.
³ Sektion Experimentelle Anaesthesiologie, University Hospital Ulm, Albert Einstein Allee 23, 89081 Ulm, Germany.
⁴ Institute of Clinical Chemistry and Pathobiochemistry, Klinikum rechts der Isar der Technische Universität München, Ismaninger Str. 22, D-81675 Munich, Germany.
⁵ Innovative Chromatography Group, Irish Separation Science Cluster (ISSC), Department of Chemistry and Analytical, Biological Chemistry Research Facility (ABCRF), University College Cork, Cork, Ireland. Electronic address: j.luong@ucc.ie.

PMID: 26717866
DOI: 10.1016/j.biotechadv.2015.12.010

Abstract

This review presents advances in assays for human C-reactive protein (CRP), the most important biomarker of infection and inflammation for a plethora of diseases and pathophysiological conditions. Routine assays in clinical settings are based on analyzers, enzyme-linked immunosorbent assays and lateral flow assays. However, assays encompassing novel sensing schemes, improved chemistry, signal enhancement, lab-on-a-chip, microfluidics and smartphone detection, have emerged in recent years. The incorporation of immune-transducing chips or sensing interfaces with nanomaterials enables multiplexing analysis of CRP with co-existing biomarkers. However, there are still considerable challenges in the development of rapid diagnostics for both pentameric and monomeric CRP forms.

Keywords: Assays; Bioanalytical advances; Biosensors; C-reactive protein; Diagnostics.

Publication types

Review

MeSH terms

C-Reactive Protein / analysis*
C-Reactive Protein / physiology
Chemistry Techniques, Analytical*
Humans

Substances

C-Reactive Protein