The RNA-seq approach for prostate cancer candidate RNA biomarkers screening in plasma and urine obtained by minimally invasive or noninvasive methods is proved to be feasible. Significant amount of RNA biomarkers associated with prostate cancer according to the literature were found in plasma and urine samples obtained from patients with benign prostatic hyperplasia (BPH). The number of detected markers was shown to vary in accordance with method of library preparation used for transcriptome profiling. The detection of known RNA biomarkers for prostate cancer in urine and plasma samples shows the feasibility of such method for minimally invasive diagnostics. The fact of presence of the same RNA biomarkers in samples from patients with BPH suggests their possible lack of specificity and confirms the need for further research in this area.
Pokazana printsipial'naia vozmozhnost' poiska kandidatnykh RNK-biomarkerov raka prostaty v biomateriale, poluchaemom maloinvazivnymi manipuliatsiiami – moche i plazme krovi. Ustanovleno, chto u patsientov s dobrokachestvennoĭ giperplazieĭ (adenomoĭ) prostaty v moche i plazme krovi obnaruzhivaetsia znachitel'noe kolichestvo RNK-biomarkerov, potentsial'no assotsiirovannykh, po literaturnym dannym, s rakom prostaty. Prodemonstrirovana zavisimost' kolichestva obnaruzhivaemykh markerov ot metoda transkriptomnogo profilirovaniia. Obnaruzhenie v issleduemykh obraztsakh plazmy krovi i mochi izvestnykh RNK-biomarkerov, assotsiirovannykh s RPZh, metodom RNA-seq pozvoliaet sdelat' vyvod o prigodnosti takogo podkhoda pri poiske novykh RNK-markerov dlia maloinvazivnoĭ diagnostiki. Pri étom sam fakt prisutstviia izvestnykh RNK-markerov RPZh v obraztsakh ot patsientov s DGPZh mozhet svidetel'stvovat' ob ikh nedostatochnoĭ spetsifichnosti i podtverzhdaet neobkhodimost' dal'neĭshikh issledovaniĭ v étoĭ oblasti.
Keywords: RNA biomarkers; RNA-seq; prostate cancer.