Objective: To investigate the anticancer efficacy and the hepatic and renal toxicity of As2O3-lipiodol emulsion via transarterial embolization in a rabbit VX2 liver tumor model.
Methods: VX2 tumors were implanted in rabbit livers successfully, followed by transarterial embolization with high-dose As2O3(5 mg/kg with 0.2 mL lipiodol, n=10), low-dose As2O3(1 mg/kg with 0.2 mL lipiodol, n=10), and control(0.2 mL lipiodol, n=10). The growth ratios and microvessel densities(MVDs) of the tumors were estimated by multi-row spiral CT and CD34 immunohistochemical staining, respectively. Hepatic and renal function was also evaluated by means of blood biochemical analysis.
Results: The growth ratios of the tumors differed significantly among three groups(P<0.01). The high-dose and low dose group showed significantly lower tumor growth ratios[44.05%(-36.40%~64.60%), 95.20%(-11.60%~159.40%)] than control group[145.55%(98.90%~250.30%), all P<0.05]. The MVDs of the tumors were significantly lower in the high-dose(21.4±10.6) and low-dose group(34.1±12.0) than those in control group(57.9±16.1,all P<0.05). The levels of blood ALT and AST obtained 28 days after transarterial embolization were significantly lower in the high-dose[(25.50±12.37)U/L,(24.25±10.89)U/L] and low-dose group[(45.00±14.04)U/L,(35.22±11.86)U/L] than in control group[(79.12±30.52)U/L,(75.25±25.89)U/L, all P<0.05].
Conclusion: As2O3-lipiodol emulsion via transarterial embolization has anticancer effect without significant hepatic and renal functional damage in rabbit VX2 liver tumors.
目的: 探讨经肝动脉三氧化二砷碘油乳化剂栓塞对兔VX2肝癌模型的抗肿瘤作用及其肝、肾功能的影响。
方法: 将30只新西兰大白兔左肝种植VX2鳞状细胞癌组织建立兔肝癌模型, 并根据肝动脉注射三氧化二砷的剂量不同分为大剂量组(碘油0.2 mL+三氧化二砷5 mg/kg)、小剂量组(碘油0.2 mL+三氧化二砷1 mg/kg)和对照组(碘油0.2 mL+0.9%氯化钠溶液2 mL)。用多排螺旋CT及CD34免疫组织化学染色法分别评价肿瘤生长率和肿瘤微血管密度, 并通过检测血清ALT、AST、尿素氮及肌酐指标评价三氧化二砷碘油乳化剂对模型兔的肝、肾毒性。
结果: 大剂量组、小剂量组的肿瘤生长率中位数分别为44.05%(-36.40%~64.60%)和95.20%(-11.60%~159.40%), 均小于对照组[145.55%(98.90%~250.30%)], 而且大剂量组小于小剂量组(均 P < 0.05)。大剂量组和小剂量组的肿瘤微血管密度分别为21.4±10.6和34.1±12.0, 均低于对照组(57.9±16.1, 均 P < 0.05)。术后28 d对照组ALT和AST水平[(79.12±30.52) U/L, (75.25±25.89) U/L]均高于大剂量组[(25.50±12.37) U/L, (24.25±10.89) U/L]和小剂量组[(45.00±14.04) U/L, (35.22±11.86) U/L, 均 P < 0.05)]; 三组间血肌酐和尿素氮水平差别均无统计学意义( P>0.05)。
结论: 经肝动脉灌注三氧化二砷碘油乳化剂对兔VX2肝癌模型具有抑制肿瘤生长和抗肿瘤血管生成的作用, 且模型未出现明显的肝、肾功能损害。