Current approaches and challenges for chemical characterization of inhibitory effect against cancer cell line isolated from Gokshur extract

Salwa Bouabdallah; Rabiaa-M Sghaier; Sawssen Selmi; Daycem Khlifi; Dhafer Laouini; Mossadok Ben-Attia

doi:10.1016/j.jchromb.2015.11.023

Current approaches and challenges for chemical characterization of inhibitory effect against cancer cell line isolated from Gokshur extract

J Chromatogr B Analyt Technol Biomed Life Sci. 2016 Jul 15:1026:279-285. doi: 10.1016/j.jchromb.2015.11.023. Epub 2015 Nov 27.

Authors

Salwa Bouabdallah¹, Rabiaa-M Sghaier², Sawssen Selmi³, Daycem Khlifi⁴, Dhafer Laouini², Mossadok Ben-Attia⁵

Affiliations

¹ Environmental Biomonitoring Laboratory LBE (LR01/ES14), Faculty of Sciences of Bizerta, University of Carthage, Tunisia. Electronic address: salwamaster@gmail.com.
² Laboratory of Transmission, Control and Immunobiology of Infection (11 LR IPT 02), Pasteur Institute, Tunisia.
³ Laboratory of Bioactive Substances, Biotechnology Centre in Borj-Cedria Technopol, BP.901, Hammam-lif, 2050, Tunisia.
⁴ Laboratory of Molecular Interactions and Chemical Reactivity and Photochemical UMR CNRS 5623, University of Toulouse, University of Paul Sabatier, 118 Route de Narbonne, F-31062 Toulouse, France.
⁵ Environmental Biomonitoring Laboratory LBE (LR01/ES14), Faculty of Sciences of Bizerta, University of Carthage, Tunisia.

PMID: 26711680
DOI: 10.1016/j.jchromb.2015.11.023

Abstract

In the present study, the potential effect anti tumor and the chemical composition of different fractions of Gokshur was evaluated. Commonly known as puncture vine, it has been used for a long time in both the Indian and traditional Chinese medicine. It is popularly used as a remedy for fertility disorder in Ayurveda. Samples were collected during June-September 2014 in the Cap Bon (north east of the northern Tunisia). Different organs were separated and extracted by sequential process to compare and investigate their potential anti-tumor effect. For the first time, we report the antiproliferatif effect of leaves n-butannolic fraction against cancer cell lines. The better anti-tumor fraction (94.76±1.52%) has been detected and performed by RP-HPLC has shown a great peak area (5578.21Mau). Novel designed natural derivatives from Gokshur, a cyclotrisiloxane, major compound identified by GC-MS.

Keywords: ATCM; Anti-cancer; Cyclotrisiloxane; GC–MS; Gokshur; MTTassay; RP-HPLC.

MeSH terms

Cell Line, Tumor
Humans
Medicine, Chinese Traditional*
Plant Extracts / pharmacology*

Substances

Plant Extracts