The complete mitogenome sequence of Glyptothorax laosensis was determined using long PCR reactions. The genome is 16 539 bp in length, including 13 typical vertebrate protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes and a control region. Except for eight tRNA and ND6 genes, all other mitochondrial genes are encoded on the heavy strand. The gene order and composition of G. laosensis was similar to that of most other vertebrates. The descending order of the base composition on heavy strand was 31.3% A, 27.5% C, 25.9% T, 15.3% G, with a relatively lower level of G and a slight AT bias of 57.2%. The results of phylogenetic reconstruction showed G. laosensis is the sister group with G. trilineatus (Figure 1), and the Glyptothorax genus formed a monophyletic group.
Keywords: Glyptothorax laosensis; mitogenome; siluriformes.