The complete chloroplast genome sequence of Maddenia hypoleuca koehne (Prunoideae, Rosaceae)

Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Nov;27(6):4651-4652. doi: 10.3109/19401736.2015.1106486. Epub 2015 Dec 28.

Abstract

Maddenia hypoleuca Koehne belonging to family Rosaceae is a native species in China. The complete chloroplast (cp) genome was generated by de novo assembly using low coverage whole genome sequencing data and manual correction. The cp genome was 158 084 bp in length, with GC content of 36.63%. It exhibited a typical quadripartite structure: a pair of large inverted repeat regions (IRs, 26 246 bp each), a large single-copy region (LSC, 86 713 bp), and a small single-copy region (SSC, 18 879 bp). A total of 114 genes were predicted, which included 80 protein-coding genes, 30 tRNA genes, and four rRNA genes. Phylogenetic analysis indicated that M. hypoleuca is most closely related to Prunus padus within the Prunoideae subfamily, which conforms to the traditional classification.

Keywords: Chloroplast genome; Illumina sequencing; Maddenia hypoleuca; phylogenetic analysis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Composition
  • Chloroplasts / genetics*
  • DNA, Chloroplast / chemistry
  • DNA, Chloroplast / isolation & purification
  • DNA, Chloroplast / metabolism
  • Genome, Chloroplast*
  • Microsatellite Repeats / genetics
  • Phylogeny
  • Plant Proteins / chemistry
  • Plant Proteins / genetics
  • Plant Proteins / metabolism
  • RNA, Ribosomal / chemistry
  • RNA, Ribosomal / genetics
  • RNA, Transfer / chemistry
  • RNA, Transfer / genetics
  • Rosaceae / classification
  • Rosaceae / genetics*
  • Sequence Analysis, DNA

Substances

  • DNA, Chloroplast
  • Plant Proteins
  • RNA, Ribosomal
  • RNA, Transfer