A diarrheic chicken simultaneously co-infected with multiple picornaviruses: Complete genome analysis of avian picornaviruses representing up to six genera

Ákos Boros; Péter Pankovics; Ádám Adonyi; Hajnalka Fenyvesi; J Michael Day; Tung Gia Phan; Eric Delwart; Gábor Reuter

doi:10.1016/j.virol.2015.12.002

A diarrheic chicken simultaneously co-infected with multiple picornaviruses: Complete genome analysis of avian picornaviruses representing up to six genera

Virology. 2016 Feb:489:63-74. doi: 10.1016/j.virol.2015.12.002. Epub 2015 Dec 18.

Authors

Ákos Boros¹, Péter Pankovics¹, Ádám Adonyi¹, Hajnalka Fenyvesi¹, J Michael Day², Tung Gia Phan³, Eric Delwart³, Gábor Reuter⁴

Affiliations

¹ Regional Laboratory of Virology, National Reference Laboratory of Gastroenteric Viruses, ÁNTSZ Regional Institute of State Public Health Service, Pécs, Hungary and University of Pécs, Pécs, Hungary.
² United States Department of Agriculture, Agricultural Research Service, U. S. National Poultry Research Center, Southeast Poultry Research Laboratory, Athens, GA, USA.
³ Blood Systems Research Institute, San Francisco, CA, USA; University of California, San Francisco, CA, USA.
⁴ Regional Laboratory of Virology, National Reference Laboratory of Gastroenteric Viruses, ÁNTSZ Regional Institute of State Public Health Service, Pécs, Hungary and University of Pécs, Pécs, Hungary. Electronic address: reuter.gabor@gmail.com.

PMID: 26707271
DOI: 10.1016/j.virol.2015.12.002

Abstract

In this study all currently known chicken picornaviruses including a novel one (chicken phacovirus 1, KT880670) were identified by viral metagenomic and RT-PCR methods from a single specimen of a diarrheic chicken suffering from a total of eight picornavirus co-infections, in Hungary. The complete genomes of six picornaviruses were determined and their genomic and phylogenetic characteristics and UTR RNA structural models analyzed in details. Picornaviruses belonged to genera Sicinivirus (the first complete genome), Gallivirus, Tremovirus, Avisivirus and "Orivirus" (two potential genotypes). In addition, the unassigned phacoviruses were also detected in multiple samples of chickens in the USA. Multiple co-infections promote and facilitate the recombination and evolution of picornaviruses and eventually could contribute to the severity of the diarrhea in chicken, in one of the most important food sources of humans.

Keywords: Bird; Chicken; Co-infection; Diarrhea; Metagenome; Picornavirus; Poultry; UTR.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

3' Untranslated Regions
5' Untranslated Regions
Amino Acid Sequence
Animals
Chickens
Coinfection / veterinary*
Coinfection / virology
Diarrhea / veterinary*
Diarrhea / virology
Genome, Viral*
Molecular Sequence Data
Nucleic Acid Conformation
Phylogeny
Picornaviridae / chemistry
Picornaviridae / classification
Picornaviridae / genetics*
Picornaviridae / physiology
Picornaviridae Infections / veterinary*
Picornaviridae Infections / virology
Poultry Diseases / virology*
RNA, Viral / chemistry
RNA, Viral / genetics
RNA, Viral / metabolism
Sequence Alignment

Substances

3' Untranslated Regions
5' Untranslated Regions
RNA, Viral

Grants and funding

NHLBI R01-HL105770/PHS HHS/United States