Rod-shaped and substituted hydroxyapatite nanoparticles stimulating type 1 and 2 cytokine secretion

Colloids Surf B Biointerfaces. 2016 Mar 1:139:10-6. doi: 10.1016/j.colsurfb.2015.12.004. Epub 2015 Dec 3.

Abstract

A Th1 immune response is required for modern vaccines as the most commonly used alum adjuvant has weak capacity for inducing Th1 immune response. Herein, rod-shaped hydroxyapatite (HA), magnesium-substituted HA (MgHA) and zinc-substituted HA (ZnHA) nanoparticles with irregular nanopores were synthesized and used as immunoadjuvants. Magnesium and zinc substitution in HA showed no influence on morphology, particle size, zeta potential and surface area of the nanoparticles. The rod-shaped MgHA and ZnHA nanoparticles promoted the cellular uptake of a molecular immunopotentiator, stimulated both type 1 and 2 cytokine secretion in vitro that relate to Th1 and Th2 immunity of bone marrow dentritic cells, respectively. The MgHA and ZnHA nanoparticles may be useful as immunoadjuvants for human.

Keywords: Adjuvant; Rod-shaped HA; Substitution; Th1 immunity; Th2 immunity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adjuvants, Immunologic / chemistry
  • Adjuvants, Immunologic / pharmacology
  • Animals
  • Bone Marrow Cells / cytology
  • Bone Marrow Cells / drug effects*
  • Bone Marrow Cells / immunology
  • Dendritic Cells / cytology
  • Dendritic Cells / drug effects*
  • Dendritic Cells / immunology
  • Durapatite / chemistry
  • Durapatite / pharmacology*
  • Femur / cytology
  • Femur / immunology
  • Interferon-gamma / biosynthesis
  • Interferon-gamma / metabolism*
  • Interleukin-4 / biosynthesis
  • Interleukin-4 / metabolism*
  • Magnesium / chemistry
  • Mice
  • Nanoparticles / chemistry*
  • Nanoparticles / ultrastructure
  • Particle Size
  • Primary Cell Culture
  • Th1-Th2 Balance / drug effects
  • Zinc / chemistry

Substances

  • Adjuvants, Immunologic
  • Interleukin-4
  • Interferon-gamma
  • Durapatite
  • Magnesium
  • Zinc