MicroRNA expression profiles of serum from patients before and after chemotherapy

Yvonne Diener; Thomas Walenda; Edgar Jost; Tim H Brümmendorf; Andreas Bosio; Wolfgang Wagner; Ute Bissels

doi:10.1016/j.gdata.2015.08.018

MicroRNA expression profiles of serum from patients before and after chemotherapy

Genom Data. 2015 Sep 2:6:125-7. doi: 10.1016/j.gdata.2015.08.018. eCollection 2015 Dec.

Authors

Yvonne Diener¹, Thomas Walenda², Edgar Jost³, Tim H Brümmendorf³, Andreas Bosio¹, Wolfgang Wagner², Ute Bissels¹

Affiliations

¹ Miltenyi Biotec GmbH, Bergisch Gladbach, Germany.
² Helmholtz Institute for Biomedical Engineering, RWTH Aachen University Medical School, Aachen, Germany.
³ Department for Hematology, Oncology, Hemostaseology and Stem Cell Transplantation, RWTH Aachen University Medical School, Aachen, Germany.

Abstract

Recovery of the blood and immune system after chemotherapy requires proliferation of hematopoietic stem and progenitor cells (HPSCs). It has been shown that systemically released factors in serum after chemotherapy stimulate HSPC expansion in vitro. We wondered if microRNAs (miRNAs) circulating in serum could account for this effect. Therefore, we compared the miRNA expression profiles of serum from patients with hematologic malignancies before and after chemotherapy. In addition to a general decrease in miRNA expression after chemotherapy, we found 23 miRNAs to be significantly differentially expressed in serum before versus after chemotherapy. The miRNA microarray data are available at NCBI's Gene Expression Omnibus (GEO) Series accession number GSE57570. Here, we provide a detailed protocol of the miRNA microarray and data analysis.

Keywords: Hematopoietic stem cells; MicroRNA; Microarray; Serum.