Ex-Vivo percutaneous absorption of enrofloxacin: Comparison of LMOG organogel vs. pentravan cream

Abstract

The objective of this study was to investigate the percutaneous absorption of enrofloxacin from two base formulations, Pentravan cream and LMOG organogel. Ex-vivo experiments were carried out on pig ear skin. The percutaneous permeation through pig skin of two formulations containing 5 wt% of enrofloxacin was measured and compared using Franz diffusion cells. At appropriate intervals up to 120 h, diffusion samples were taken and analyzed using HPLC assays. Permeation profiles were established and the parameters Tlag and flux values were calculated. In this ex-vivo study, the flux values were 0.35 μgcm(-2)h(-1) for Pentravan and 1.22 μgcm(-2)h(-1) for LMOG organogel, corresponding respectively to 7.9 % and 29.3 % of enrofloxacin absorbed after 120 h by these formulations. The lag time (T lag) of Pentravan and organogel were 6.32 and 0.015 h respectively. The absorption time to reach the antibiotic concentration of enrofloxacin (2 μgmL(-1)) in the receptor was 60 h with Pentravan and 30 h with the organogel, suggesting more effective treatment by the latter. Enrofloxacin contained in organogel could be absorbed through pig ear skin 3.7 times greater than that in Pentravan (commercial formulation). This study demonstrates the perspective of organogel formulations as potential drug delivery systems.

Keywords: 12-hydroxystearic acid (PubChem CID: 7789); Demineralized water (PubChem CID: 962); Enrofloxacin; Enrofloxacin (PubChem CID: 71188); Glycerol (PubChem CID: 753); HPLC quantification; In-vitro percutaneous absorption; Isopropyl myristate (PubChem CID: 8042); LMOG organogel; Oleic acid (PubChem CID: 445639); Pentravan(®); Perchloric acid (PubChem CID: 24247); Phosphate-Buffered Saline (PubChem CID: 249785514); Polyoxyl 40 stearate (PubChem CID: 24762); Simethicone (PubChem CID: 6433516); Soja lecithin (PubChem CID: 57369748).