Detection and identification of pathotypes of verocytotoxigenic Escherichia coli isolated from weaned piglets using gene probes for seven E. coli toxins

FEMS Microbiol Lett. 1989 Jun;50(3):345-9. doi: 10.1016/0378-1097(89)90443-6.

Abstract

Seventy verocytotoxigenic (VTEC) and sixty-three non VTEC haemolytic Escherichia coli isolated from recently weaned piglets were examined by the colony hybridization assay using gene probes for three verocytotoxins: Edema disease principle (EDP) and Shiga-like toxins I and II (SLTI and SLTII). The results with the EDP and SLTII probes were identical. All VTEC hybridized with these two probes, while non VTEC did not. All 133 E. coli were negative for the SLTI probe. Hybridization of the plasmid content of 14 VTEC did not show any evidence for plasmid localization of the genes coding for the EDP. The 70 VTEC were also assayed with gene probes for heat-stable (STaP, STb) and heat-labile (LT, LTIIa) enterotoxins. Only the STb probe was hybridized by 36 of them. Most STb-positive isolates belonged to serotype O141: K85 biotypes 9 and 13 PC.

MeSH terms

  • Animals
  • Bacterial Toxins / genetics
  • DNA Probes
  • Edema Disease of Swine / microbiology*
  • Escherichia coli / classification
  • Escherichia coli / genetics
  • Escherichia coli / pathogenicity
  • Escherichia coli Infections / microbiology
  • Escherichia coli Infections / veterinary*
  • Shiga Toxin 1
  • Swine

Substances

  • Bacterial Toxins
  • DNA Probes
  • Shiga Toxin 1