Objective: To investigate the effect of Suoquan Wan(SQW) and Jinkui Shenqi Wan(JKSQW) on urethra function and β6-AR function of detrusor in natural aged rats.
Methods: young rats(3 months) and Aged rats(15 months) were chosen. Young rats were chosen as control, aged rats were randomly divided into three groups: model, JKSQW and SQW groups, 12 rats in each group. JKSQW and SQW were given the appropriate concentration once a day for six weeks. The effects of SQW and JKSQW on relaxation function of bladder detrusor was investigated, and their effects on bladder induced by β3-AR agonist and β3-AR agonist were further studied. Then, their effects on pressure of urethra were observed.
Results: Compared with the young group, detrusor compliance of natural aged model rats was increased, Emax and IA of agonist of β3-AR including BRL37344 and ISO were decreased(P <0. 01), while PA2 of antagonist of β3-AR were increased(P <0. 05). Compared with model group, SQW and JKSQW decreased the bladder compliance(P <0. 05), and increased Emax, IA and PD2 due to sensitivity of detrusor to agonist of β3-AR and β3-AR including BRL37344 and ISO(P <0. 05 or P < 0. 01) ,while decreased PA2 of antagonist of β3-AR(P <0. 05). MUCP, MUP and FUL of aged rats were lower than those of normal rats. But SQW and JKSQW increased MUCP and MUP, and JKSQW increased FUL of aged rats(P <0. 05 or P <0. 01).
Conclusion: SQW and JKSQW can remarkably adjust β3-AR function on the detrusor and improve the closure ability of bladder detrusor of the natural aged rats.