Biliary tract external drainage protects against intestinal barrier injury in hemorrhagic shock rats

World J Gastroenterol. 2015 Dec 7;21(45):12800-13. doi: 10.3748/wjg.v21.i45.12800.

Abstract

Aim: To investigate the effects of biliary tract external drainage (BTED) on intestinal barrier injury in rats with hemorrhagic shock (HS).

Methods: BTED was performed via cannula insertion into the bile duct of rats. HS was induced by drawing blood from the femoral artery at a rate of 1 mL/min until a mean arterial pressure (MAP) of 40 ± 5 mmHg was achieved. That MAP was maintained for 60 min. A total of 99 Sprague-Dawley rats were randomized into a sham group, an HS group and an HS + BTED group. Nine rats in the sham group were sacrificed 0.5 h after surgery. Nine rats in each of the HS and HS + BTED groups were sacrificed 0.5 h, 1 h, 2 h, 4 h and 6 h after resuscitation. Plasma tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and lipopolysaccharide (LPS) levels were analyzed using enzyme-linked immunosorbent assay. Plasma D-lactate levels were analyzed using colorimetry. The expression levels of occludin and claudin-1 in the ileum were analyzed using Western blot and immunohistochemistry. Histology of the ileum was evaluated by hematoxylin and eosin staining.

Results: Plasma TNF-α levels in the HS + BTED group decreased significantly compared with the HS group at 1 h and 6 h after resuscitation (P < 0.05). Plasma IL-6 levels in the HS + BTED group decreased significantly compared with the HS group at 0.5 h, 1 h and 2 h after resuscitation (P < 0.05). Plasma D-lactate and LPS levels in the HS + BTED group decreased significantly compared with the HS group at 6 h after resuscitation (P < 0.05). The expression levels of occludin in the HS + BTED group increased significantly compared with the HS group at 4 h and 6 h after resuscitation (P < 0.05). The expression levels of claudin-1 in the HS + BTED group increased significantly compared with the HS group at 6 h after resuscitation (P < 0.05). Phenomena of putrescence and desquamation of epithelial cells in the ileal mucosa were attenuated in the HS + BTED group. Ileal histopathologic scores in the HS + BTED group decreased significantly compared with the HS group at 2 h, 4 h and 6 h after resuscitation (P < 0.05).

Conclusion: BTED protects against intestinal barrier injury in HS rats.

Keywords: Biliary tract external drainage; Claudin-1; D-lactate; Hemorrhagic shock; Occludin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biliary Tract Surgical Procedures / methods*
  • Biomarkers / blood
  • Blotting, Western
  • Claudin-1 / metabolism
  • Disease Models, Animal
  • Drainage / methods*
  • Ileum / metabolism
  • Ileum / pathology*
  • Immunohistochemistry
  • Inflammation Mediators / blood
  • Interleukin-6 / blood
  • Lactic Acid / blood
  • Lipopolysaccharides / blood
  • Male
  • Occludin / metabolism
  • Rats, Sprague-Dawley
  • Shock, Hemorrhagic / blood
  • Shock, Hemorrhagic / pathology
  • Shock, Hemorrhagic / surgery*
  • Time Factors
  • Tumor Necrosis Factor-alpha / blood

Substances

  • Biomarkers
  • Claudin-1
  • Cldn1 protein, rat
  • Inflammation Mediators
  • Interleukin-6
  • Lipopolysaccharides
  • Occludin
  • Ocln protein, rat
  • Tumor Necrosis Factor-alpha
  • Lactic Acid