One-pot preparation of labelled mannan-peptide conjugate, model for immune cell processing

Glycoconj J. 2016 Feb;33(1):113-20. doi: 10.1007/s10719-015-9644-0. Epub 2015 Dec 14.

Abstract

An efficient method for preparation of fluorescently labelled mannan-peptide glycoconjugates has been developed. After selective Dess-Martin periodinane oxidation of mannan, it was conjugated to the fluorescent label alone and a peptide with the label via reductive amination. Prepared glycoconjugates were characterised by HPSEC, FTIR-ATR and UV-VIS spectroscopy. Finally, the fluorescently labelled mannan and mannan-peptide conjugate were used for microscopic visualization of their accumulation in intracellular organelles of RAW 264.7 cells.

Keywords: 2-aminobenzamide; Candida albicans; Fluorescent label; Glycoconjugate; Mannan.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Candida / chemistry
  • Cell Line
  • Fungal Polysaccharides / chemistry*
  • Fungal Polysaccharides / immunology
  • Macrophages / immunology
  • Mice
  • Peptides / chemistry*
  • Peptides / immunology
  • Vaccines, Conjugate / chemistry*
  • Vaccines, Conjugate / immunology

Substances

  • Fungal Polysaccharides
  • Peptides
  • Vaccines, Conjugate