Highly efficient generation of biallelic reporter gene knock-in mice via CRISPR-mediated genome editing of ESCs

Protein Cell. 2016 Feb;7(2):152-6. doi: 10.1007/s13238-015-0228-3.

Authors

Yanliang Wang¹, Junhong Li¹, Jinzhu Xiang¹, Bingqiang Wen¹, Haiyuan Mu¹, Wei Zhang¹, Jianyong Han²

Affiliations

¹ State Key Laboratories for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, 100083, China.
² State Key Laboratories for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, 100083, China. hanjy@cau.edu.cn.

No abstract available

Publication types

Letter
Research Support, Non-U.S. Gov't

MeSH terms

Alleles*
Animals
Clustered Regularly Interspaced Short Palindromic Repeats / genetics*
Embryonic Stem Cells / metabolism*
Gene Knock-In Techniques / methods*
Genes, Reporter / genetics*
Genetic Engineering / methods*
Genomics*
Mice