Inhibitory effect of polyozellin on secretory group IIA phospholipase A2

Arch Pharm Res. 2016 Feb;39(2):271-278. doi: 10.1007/s12272-015-0694-4. Epub 2015 Dec 10.

Abstract

The expression of secretory group IIA phospholipase A2 (sPLA2-IIA) is enhanced by development of inflammatory disorders. In this study, sPLA2-IIA expression was induced in the lipopolysaccharide (LPS)-stimulated human umbilical vein endothelial cells and mice to evaluate the effect of polyozellin. Polyozellin, a major constituent of a Korea edible mushroom Polyozellus multiplex, has been known to exhibit the biological activities such as anti-oxidative and anti-inflammatory effects. Polyozellin remarkably suppressed the LPS-mediated protein expression and activity of sPLA2-IIA via inhibition of phosphorylation of cytosolic phospholipase A2 and extracellular signal-regulated kinase 1/2. These results demonstrated that polyozellin might play an important role in the modulation of sPLA2-IIA expression and activity in response to the inflammatory diseases.

Keywords: HUVECs; Inflammation; Polyozellin; sPLA2-IIA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Agaricales / chemistry
  • Animals
  • Anti-Inflammatory Agents / isolation & purification
  • Anti-Inflammatory Agents / pharmacology*
  • Cells, Cultured
  • Cyclooxygenase 2 / metabolism
  • Disease Models, Animal
  • Dose-Response Relationship, Drug
  • Enzyme Inhibitors / isolation & purification
  • Enzyme Inhibitors / pharmacology*
  • Extracellular Signal-Regulated MAP Kinases / metabolism
  • Furans / isolation & purification
  • Furans / pharmacology*
  • Group II Phospholipases A2 / antagonists & inhibitors*
  • Group II Phospholipases A2 / metabolism
  • Human Umbilical Vein Endothelial Cells / drug effects*
  • Human Umbilical Vein Endothelial Cells / enzymology
  • Lipopolysaccharides / pharmacology
  • Male
  • Mice, Inbred ICR
  • Phosphorylation
  • Prostaglandin-E Synthases / metabolism
  • Sepsis / drug therapy*
  • Sepsis / enzymology
  • Sepsis / microbiology
  • Time Factors

Substances

  • Anti-Inflammatory Agents
  • Enzyme Inhibitors
  • Furans
  • Lipopolysaccharides
  • polyozellin
  • Cyclooxygenase 2
  • PTGS2 protein, human
  • Extracellular Signal-Regulated MAP Kinases
  • Group II Phospholipases A2
  • PLA2G2A protein, human
  • Pla2g2a protein, mouse
  • PTGES protein, human
  • Prostaglandin-E Synthases