Abstract
The expression of secretory group IIA phospholipase A2 (sPLA2-IIA) is enhanced by development of inflammatory disorders. In this study, sPLA2-IIA expression was induced in the lipopolysaccharide (LPS)-stimulated human umbilical vein endothelial cells and mice to evaluate the effect of polyozellin. Polyozellin, a major constituent of a Korea edible mushroom Polyozellus multiplex, has been known to exhibit the biological activities such as anti-oxidative and anti-inflammatory effects. Polyozellin remarkably suppressed the LPS-mediated protein expression and activity of sPLA2-IIA via inhibition of phosphorylation of cytosolic phospholipase A2 and extracellular signal-regulated kinase 1/2. These results demonstrated that polyozellin might play an important role in the modulation of sPLA2-IIA expression and activity in response to the inflammatory diseases.
Keywords:
HUVECs; Inflammation; Polyozellin; sPLA2-IIA.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Agaricales / chemistry
-
Animals
-
Anti-Inflammatory Agents / isolation & purification
-
Anti-Inflammatory Agents / pharmacology*
-
Cells, Cultured
-
Cyclooxygenase 2 / metabolism
-
Disease Models, Animal
-
Dose-Response Relationship, Drug
-
Enzyme Inhibitors / isolation & purification
-
Enzyme Inhibitors / pharmacology*
-
Extracellular Signal-Regulated MAP Kinases / metabolism
-
Furans / isolation & purification
-
Furans / pharmacology*
-
Group II Phospholipases A2 / antagonists & inhibitors*
-
Group II Phospholipases A2 / metabolism
-
Human Umbilical Vein Endothelial Cells / drug effects*
-
Human Umbilical Vein Endothelial Cells / enzymology
-
Lipopolysaccharides / pharmacology
-
Male
-
Mice, Inbred ICR
-
Phosphorylation
-
Prostaglandin-E Synthases / metabolism
-
Sepsis / drug therapy*
-
Sepsis / enzymology
-
Sepsis / microbiology
-
Time Factors
Substances
-
Anti-Inflammatory Agents
-
Enzyme Inhibitors
-
Furans
-
Lipopolysaccharides
-
polyozellin
-
Cyclooxygenase 2
-
PTGS2 protein, human
-
Extracellular Signal-Regulated MAP Kinases
-
Group II Phospholipases A2
-
PLA2G2A protein, human
-
Pla2g2a protein, mouse
-
PTGES protein, human
-
Prostaglandin-E Synthases