Development and Validation of a PCR Assay To Detect the Prairie Epidemic Strain of Pseudomonas aeruginosa from Patients with Cystic Fibrosis

M Workentine; A Poonja; B Waddell; J Duong; D G Storey; D Gregson; R Somayaji; H R Rabin; M G Surette; M D Parkins

doi:10.1128/JCM.02603-15

Development and Validation of a PCR Assay To Detect the Prairie Epidemic Strain of Pseudomonas aeruginosa from Patients with Cystic Fibrosis

J Clin Microbiol. 2016 Feb;54(2):489-91. doi: 10.1128/JCM.02603-15. Epub 2015 Dec 9.

Authors

M Workentine¹, A Poonja², B Waddell², J Duong³, D G Storey⁴, D Gregson⁵, R Somayaji⁵, H R Rabin⁵, M G Surette⁶, M D Parkins⁷

Affiliations

¹ Department of Veterinary Medicine, University of Calgary, Calgary, Canada.
² Department of Microbiology, Immunology, and Infectious Diseases, University of Calgary, Calgary, Canada.
³ Department of Biological Sciences, University of Calgary, Calgary, Canada.
⁴ Department of Microbiology, Immunology, and Infectious Diseases, University of Calgary, Calgary, Canada Department of Biological Sciences, University of Calgary, Calgary, Canada.
⁵ Department of Microbiology, Immunology, and Infectious Diseases, University of Calgary, Calgary, Canada Department of Medicine, University of Calgary, Calgary, Canada.
⁶ Department of Microbiology, Immunology, and Infectious Diseases, University of Calgary, Calgary, Canada Department of Medicine, McMaster University, Hamilton, Ontario, Canada Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada The Farncombe Family Digestive Health Research Institute, McMaster University, Hamilton, Ontario, Canada.
⁷ Department of Microbiology, Immunology, and Infectious Diseases, University of Calgary, Calgary, Canada Department of Medicine, University of Calgary, Calgary, Canada mdparkin@ucalgary.ca.

Abstract

The monitoring of epidemic Pseudomonas aeruginosa is important for cystic fibrosis (CF) infection control. The prairie epidemic strain (PES) is common in western Canadian CF clinics. Using whole-genome sequencing, we identified a novel genomic island and developed a PCR assay for PES. Against a collection of 186 P. aeruginosa isolates, the assay had 98% sensitivity and 100% specificity.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cystic Fibrosis / complications*
Electrophoresis, Gel, Pulsed-Field
Genome, Bacterial
Humans
Multilocus Sequence Typing / methods
Polymerase Chain Reaction* / methods
Pseudomonas Infections / diagnosis*
Pseudomonas Infections / microbiology*
Pseudomonas aeruginosa / classification*
Pseudomonas aeruginosa / genetics*
Random Amplified Polymorphic DNA Technique
Sensitivity and Specificity