High-Frequency, Low-Intensity Pulsed Ultrasound Enhances Alveolar Bone Healing of Extraction Sockets in Rats: A Pilot Study

Kyung Lhi Kang; Eun-Cheol Kim; Joon Bong Park; Jung Sun Heo; Yumi Choi

doi:10.1016/j.ultrasmedbio.2015.10.022

High-Frequency, Low-Intensity Pulsed Ultrasound Enhances Alveolar Bone Healing of Extraction Sockets in Rats: A Pilot Study

Ultrasound Med Biol. 2016 Feb;42(2):493-502. doi: 10.1016/j.ultrasmedbio.2015.10.022. Epub 2015 Nov 30.

Authors

Kyung Lhi Kang¹, Eun-Cheol Kim², Joon Bong Park³, Jung Sun Heo⁴, Yumi Choi⁵

Affiliations

¹ Department of Periodontology, School of Dentistry, Kyung Hee University, Seoul, Korea; Institute of Oral Biology, School of Dentistry, Kyung Hee University, Seoul, Korea. Electronic address: periokkl@khu.ac.kr.
² Institute of Oral Biology, School of Dentistry, Kyung Hee University, Seoul, Korea; Department of Oral and Maxillofacial Pathology, School of Dentistry and Research Center for Tooth & Periodontal Tissue Regeneration (MRC), Kyung Hee University, Seoul, Korea.
³ Department of Periodontology, School of Dentistry, Kyung Hee University, Seoul, Korea; Institute of Oral Biology, School of Dentistry, Kyung Hee University, Seoul, Korea.
⁴ Institute of Oral Biology, School of Dentistry, Kyung Hee University, Seoul, Korea; Department of Maxillofacial Biomedical Engineering, School of Dentistry, Kyung Hee University, Seoul, Korea.
⁵ Department of Periodontology, School of Dentistry, Kyung Hee University, Seoul, Korea.

PMID: 26653935
DOI: 10.1016/j.ultrasmedbio.2015.10.022

Abstract

Most studies of the beneficial effects of low-intensity pulsed ultrasound (LIPUS) on bone healing have used frequencies between 1.0 and 1.5 MHz. However, after consideration of ultrasound wave characteristics and depth of target tissue, higher-frequency LIPUS may have been more effective on superficially positioned alveolar bone. We investigated this hypothesis by applying LIPUS (frequency, 3.0 MHz; intensity, 30 mW/cm(2)) on shaved right cheeks over alveolar bones of tooth extraction sockets in rats for 10 min/d for 2 wk after tooth extraction; the control group (left cheek of the same rats) did not receive LIPUS treatment. Compared with the control group, the LIPUS group manifested more new bone growth inside the sockets on histomorphometric analysis (maximal difference = 2.5-fold on the seventh day after extraction) and higher expressions of osteogenesis-related mRNAs and proteins than the control group did. These findings indicate that 3.0-MHz LIPUS could enhance alveolar bone formation and calcification in rats.

Keywords: Alveolar bone formation in rats; High frequency; Low-intensity pulsed ultrasound; Tooth extraction socket healing.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bone Regeneration / radiation effects*
Dose-Response Relationship, Drug
Male
Molar / pathology
Molar / radiation effects
Molar / surgery*
Pilot Projects
Radiation Dosage
Rats
Rats, Sprague-Dawley
Tooth Extraction*
Tooth Socket / growth & development*
Tooth Socket / pathology
Tooth Socket / radiation effects*
Treatment Outcome
Ultrasonic Therapy / methods*
Ultrasonic Waves