Elevated plasma-soluble CD16 levels in porcine reproductive and respiratory syndrome virus-infected pigs: correlation with ADAM17-mediated shedding

J Gen Virol. 2016 Mar;97(3):632-638. doi: 10.1099/jgv.0.000368. Epub 2015 Dec 10.

Abstract

Soluble CD16 (sCD16) is closely correlated with chronic diseases in humans. Here, plasma sCD16 levels in pigs were increased by infection with porcine reproductive and respiratory syndrome virus (PRRSV) but not with porcine epidemic diarrhea virus, porcine circovirus type 2 and pseudorabies virus. Of interest, PRRSV attached to blood neutrophils and reduced surface CD16 expression on neutrophils. In vitro data confirmed that PRRSV caused CD16 shedding in neutrophils. Further analyses revealed that ADAM17 was involved in porcine CD16 shedding. Thus, our findings suggest that increase in sCD16 levels may be an indicator of PRRSV infection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ADAM Proteins / genetics
  • ADAM Proteins / metabolism*
  • ADAM17 Protein
  • Animals
  • Host-Pathogen Interactions
  • Neutrophils / metabolism
  • Neutrophils / virology
  • Porcine Reproductive and Respiratory Syndrome / blood
  • Porcine Reproductive and Respiratory Syndrome / enzymology*
  • Porcine Reproductive and Respiratory Syndrome / genetics
  • Porcine Reproductive and Respiratory Syndrome / virology
  • Porcine respiratory and reproductive syndrome virus / genetics
  • Porcine respiratory and reproductive syndrome virus / physiology*
  • Receptors, IgG / blood*
  • Receptors, IgG / genetics
  • Sus scrofa
  • Swine

Substances

  • Receptors, IgG
  • ADAM Proteins
  • ADAM17 Protein
  • ADAM17 protein, human