Microwave Heating of Poly(N-isopropylacrylamide)-Conjugated Gold Nanoparticles for Temperature-Controlled Display of Concanavalin A

Roshan B Vasani; Nayana Janardanan; Beatriz Prieto-Simón; Anna Cifuentes-Rius; Siobhan J Bradley; Eli Moore; Tobias Kraus; Nicolas H Voelcker

doi:10.1021/acsami.5b08765

Microwave Heating of Poly(N-isopropylacrylamide)-Conjugated Gold Nanoparticles for Temperature-Controlled Display of Concanavalin A

ACS Appl Mater Interfaces. 2015 Dec 23;7(50):27755-64. doi: 10.1021/acsami.5b08765. Epub 2015 Dec 14.

Authors

Roshan B Vasani¹, Nayana Janardanan¹, Beatriz Prieto-Simón¹, Anna Cifuentes-Rius¹, Siobhan J Bradley², Eli Moore¹, Tobias Kraus³, Nicolas H Voelcker¹

Affiliations

¹ ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, Future Industries Institute, University of South Australia , Mawson Lakes, South Australia 5095, Australia.
² Future Industries Institute, University of South Australia , Mawson Lakes, South Australia 5095, Australia.
³ INM-Leibniz Institute for New Materials , Campus D2 2, Saarbruecken, Saarland 66123, Germany.

PMID: 26629977
DOI: 10.1021/acsami.5b08765

Abstract

We demonstrate microwave-induced heating of gold nanoparticles and nanorods. An appreciably higher and concentration-dependent microwave-induced heating rate was observed with aqueous dispersions of the nanomaterials as opposed to pure water and other controls. Grafted with the thermoresponsive polymer poly(N-isopropylacrylamide), these gold nanomaterials react to microwave-induced heating with a conformational change in the polymer shell, leading to particle aggregation. We subsequently covalently immobilize concanavalin A (Con A) on the thermoresponsive gold nanoparticles. Con A is a bioreceptor commonly used in bacterial sensors because of its affinity for carbohydrates on bacterial cell surfaces. The microwave-induced thermal transitions of the polymer reversibly switch on and off the display of Con A on the particle surface and hence the interactions of the nanomaterials with carbohydrate-functionalized surfaces. This effect was determined using linear sweep voltammetry on a methyl-α-d-mannopyranoside-functionalized electrode.

Keywords: concanavalin A; controlled display; gold nanoparticles; microwave heating; poly(N-isopropylacrylamide).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acrylic Resins / chemical synthesis
Acrylic Resins / chemistry*
Concanavalin A / chemical synthesis
Concanavalin A / chemistry*
Gold / chemistry
Metal Nanoparticles / chemistry*
Microwaves
Particle Size
Surface Properties
Temperature

Substances

Acrylic Resins
Concanavalin A
poly-N-isopropylacrylamide
Gold