In this unit, the fully automated solid-phase synthetic strategy of hairpin Py-Im polyamides is described using triphosgene (BTC) as a coupling agent. This automated methodology is compatible with all the typical building blocks, enabling the facile synthesis of polyamide libraries in 9% to 20% yield in 3 days.
Keywords: DNA; Py-Im polyamide; automated synthesis; minor groove; solid-phase synthesis; triphosgene.
Copyright © 2015 John Wiley & Sons, Inc.