Lipoic acid reduces inflammation in a mouse focal cortical experimental autoimmune encephalomyelitis model

J Neuroimmunol. 2015 Dec 15:289:68-74. doi: 10.1016/j.jneuroim.2015.10.011. Epub 2015 Nov 11.

Abstract

Cortical lesions are a crucial part of MS pathology and it is critical to determine that new MS therapies have the ability to alter cortical inflammatory lesions given the differences between white and gray matter lesions. We tested lipoic acid (LA) in a mouse focal cortical EAE model. Brain sections were stained with antibodies against CD4, CD11b and galectin-3. Compared with vehicle, treatment with LA significantly decreased CD4+ and galectin-3+ immune cells in the brain. LA treated mice had fewer galectin-3+ cells with no projections indicating decrease in the number of infiltrating monocytes. LA significantly reduces inflammation in a focal cortical model of MS.

Keywords: Cortical lesions; Focal EAE; Immunofluorescence; Inflammation; Multiple sclerosis.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Antioxidants / therapeutic use*
  • CD11b Antigen / metabolism
  • Cerebral Cortex / drug effects
  • Cerebral Cortex / pathology*
  • Cytokines / metabolism
  • Cytokines / pharmacology
  • Disease Models, Animal
  • Encephalitis / drug therapy*
  • Encephalitis / etiology*
  • Encephalomyelitis, Autoimmune, Experimental / chemically induced
  • Encephalomyelitis, Autoimmune, Experimental / complications*
  • Encephalomyelitis, Autoimmune, Experimental / pathology*
  • Female
  • Galectin 3 / metabolism
  • Mice
  • Mice, Inbred C57BL
  • Myelin-Oligodendrocyte Glycoprotein / immunology
  • Peptide Fragments / immunology
  • Statistics, Nonparametric
  • Thioctic Acid / therapeutic use*
  • Time Factors

Substances

  • Antioxidants
  • CD11b Antigen
  • Cytokines
  • Galectin 3
  • Myelin-Oligodendrocyte Glycoprotein
  • Peptide Fragments
  • myelin oligodendrocyte glycoprotein (35-55)
  • Thioctic Acid